The effects of human milk proteins on the proliferation of normal, cancer and cancer stem like cells
- Authors
- Kang, Nam Mi; Cho, Ssang-Goo; Dayem, Ahmed Abdal; Lee, Joohyun; Bae, Seong Phil; Hahn, Won-Ho; Lee, Jeong-Sang
- Issue Date
- Dec-2018
- Publisher
- 한국분석과학회
- Keywords
- human breast milk; beta-casein; kappa-casein; lactoferrin; Cell-proliferation; breast cancer cell
- Citation
- 분석과학, v.31, no.6, pp 232 - 239
- Pages
- 8
- Journal Title
- 분석과학
- Volume
- 31
- Number
- 6
- Start Page
- 232
- End Page
- 239
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/5436
- DOI
- 10.5806/AST.2018.31.6.232
- ISSN
- 1225-0163
2288-8985
- Abstract
- Human breast milk (HBM) provides neonates with indispensable nutrition. The present study evaluated the anti-cancer activity of diluted and pasteurized early HBM (< 6 weeks' lactation) on human breast cancer cell lines. The cell lines MCF7 and MDA-MB231 were exposed to 1% HBM from the 1st, 3rd, and 6th weeks of lactation and exhibited reduced proliferation rates. As controls, breast cell lines (293T and MCF-10A), breast cancer cell lines (MCF-7 and MDA-MB-231), and CD133(hi)CXCR4(hi)ALDH1(hi) patient-derived human cancer stem-like cells (KU-CSLCs) were treated with prominent milk proteins beta-casein, kappa-casein, and lactoferrin at varying doses (10, 50, and 100 mu g) for 24 or 48 hrs. The impact of these proteins on cell proliferation was investigated. Breast cancer cell lines treated with kappa-casein and lactoferrin exhibited significantly reduced viability, in both a dose- and time-dependent manner. Interestingly, kappa-casein selectively impacted only cancer (but not normal breast) cell lines, particularly the more malignant cell line. However, beta-casein-exposed human breast cancer cell lines exhibited a significantly higher proliferation rate. Thus, kappa-casein and lactoferrin appear to exert selective anti-cancer activities. Further studies are warranted to determine the mechanisms underlying kappa-casein- and lactoferrin-mediated cancer cell-selective cytotoxic effects.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Pediatrics > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.