Phosphatidylethanolamine-binding protein 1 protects CA1 neurons against ischemic damage via ERK-CREB signaling in Mongolian gerbils
DC Field | Value | Language |
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dc.contributor.author | Jung, Hyo Young | - |
dc.contributor.author | Cho, Su Bin | - |
dc.contributor.author | Kim, Woosuk | - |
dc.contributor.author | Yoo, Dae Young | - |
dc.contributor.author | Won, Moo-Ho | - |
dc.contributor.author | Choi, Goang-Min | - |
dc.contributor.author | Cho, Tack-Geun | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Hwang, In Koo | - |
dc.contributor.author | Choi, Soo Young | - |
dc.contributor.author | Moon, Seung Myung | - |
dc.date.accessioned | 2021-08-11T11:43:52Z | - |
dc.date.available | 2021-08-11T11:43:52Z | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 0197-0186 | - |
dc.identifier.issn | 1872-9754 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/5662 | - |
dc.description.abstract | In the present study, we made a PEP-1-phosphatidylethanolamine-binding protein 1 (PEP-1-PEBP1) fusion protein to facilitate the transduction of PEBP1 into cells and observed significant ameliorative effects of PEP-1-PEBP1 against H2O2-induced neuronal damage and the formation of reactive oxygen species in the HT22 hippocampal cells. In addition, administration of PEP-1-PEBP1 fusion protein ameliorated H2O2-induced phosphorylation of extracellular signal-regulated kinases (ERK1/2) and facilitated the phosphorylation of cyclic-AMP response element binding protein (CREB) in HT22 cells after exposure to H2O2. We also investigated the temporal and spatial changes of phosphorylated phosphatidylethanolamine-binding protein 1 (pPEBP1) in the hippocampus, after 5 min of transient forebrain ischemia in gerbils. In the sham-operated animals, pPEBP1 immunoreactivity was not detectable in the hippocampal CA1 region. pPEBP1 immunoreactivity was significantly increased in the hippocampal CA1 region, 1-2 days after ischemia, compared to that in the sham operated group and pPEBP1 immunoreactivity was returned to levels in sham-operated group at 3-4 days after ischemia. pPEBP1 immunoreactivity significantly increased at day 7 after ischemia and decreased to sham operated group levels by day 10 after ischemia/reperfusion. In addition, administration of PEP-1-PEBP1 fusion protein significantly reduced the ischemia-induced hyperactivity of locomotion, 1 day after ischemia and PEP-1-PEBP1 reduced neuronal damage and reactive gliosis (astrocytosis and microgliosis) in the gerbil hippocampal CA1 region, 4 days after ischemia. Administration of PEP-1-PEBP1 fusion protein ameliorated the ischemia-induced phosphorylation of ERK at 3 h and 6 h after ischemia/reperfusion and accelerated the phosphorylation of CREB in ischemic hippocampus at 6 h after ischemia. These results suggest that the increase in PEBP1 phosphorylation causes neuronal damage in the hippocampus and treatment with PEP-1-PEBP1 fusion protein provides neuroprotection from increasing phosphorylation of ERK-CREB pathways in the hippocampal CA1 region, during ischemic damage. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Phosphatidylethanolamine-binding protein 1 protects CA1 neurons against ischemic damage via ERK-CREB signaling in Mongolian gerbils | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.neuint.2018.05.005 | - |
dc.identifier.scopusid | 2-s2.0-85047069729 | - |
dc.identifier.wosid | 000439674200029 | - |
dc.identifier.bibliographicCitation | Neurochemistry International, v.118, pp 265 - 274 | - |
dc.citation.title | Neurochemistry International | - |
dc.citation.volume | 118 | - |
dc.citation.startPage | 265 | - |
dc.citation.endPage | 274 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | NEUROSTIMULATING PEPTIDE PRECURSOR | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | SYNAPTIC PLASTICITY | - |
dc.subject.keywordPlus | CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | ALZHEIMERS-DISEASE | - |
dc.subject.keywordPlus | CELL-DAMAGE | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | HIPPOCAMPUS | - |
dc.subject.keywordAuthor | Phosphatidylethanolamine-binding protein 1 | - |
dc.subject.keywordAuthor | Hippocampus | - |
dc.subject.keywordAuthor | Gerbil | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | PEP-1 fusion protein | - |
dc.subject.keywordAuthor | MAPK | - |
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