Transduction Patterns of Adeno-associated Viral Vectors in a Laser-Induced Choroidal Neovascularization Mouse Model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Si Hyung | - |
dc.contributor.author | Kim, Ye Seul | - |
dc.contributor.author | Nah, Seung Kwan | - |
dc.contributor.author | Kim, Hee Jong | - |
dc.contributor.author | Park, Ha Yan | - |
dc.contributor.author | Yang, Jin Young | - |
dc.contributor.author | Park, Keerang | - |
dc.contributor.author | Park, Tae Kwann | - |
dc.date.accessioned | 2021-08-11T12:23:49Z | - |
dc.date.available | 2021-08-11T12:23:49Z | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 2329-0501 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/5944 | - |
dc.description.abstract | Adeno-associated virus (AAV) vector is a promising platform technology for ocular gene therapy. Recently clinical successes to treat choroidal neovascularization (CNV) in wet type age-related macular degeneration have been reported. However, because pathologic conditions of the retina may alter the tropism of viral vectors, it is necessary to evaluate the transduction efficiency of different serotypes of AAV vectors in the retinas with CNVs. Here, we show the patterns and efficacy of transduction of AAV2, -5, and -8 vectors in a laser-induced CNV mouse model. C57BL/6J mice were subjected to unilateral laser photocoagulation on the right eye to induce CNV 5 days prior to intravitreal injection of AAV2, -5, and -8 capsids expressing EGFP. Transduction was increased around CNV lesions for all AAV capsid types, and AAV2 resulted in the highest transduction efficiency. In the absence of CNV, the AAV2 vector transduced ganglion and inner nuclear layer (INL) cells, and AAV5 and AAV8 transduced only a small proportion of cells in the retinal ganglion cell layer. CNV increased AAV2 vector expression throughout the retina and in and around CNVs; the transduced cells included retinal ganglion cells, Muller cells, cells from the INL and outer nuclear layer (ONL), photoreceptors, and retinal pigment epithelium (RPE) cells. Inflammatory cells and endothelial cells in CNVs were also transduced by AAV2. AAV5 and AAV8 were transduced in retinal ganglion, Muller, INL, ONL, and RPE cells in a localized pattern, and only endothelial cells at the surface of CNV lesions showed EGFP expression. Taken together, CNV formation resulted in enhanced transduction of AAV2, -5, and -8, and AAV2 exhibited the highest transduction efficiency in cells in CNV lesions. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Transduction Patterns of Adeno-associated Viral Vectors in a Laser-Induced Choroidal Neovascularization Mouse Model | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.omtm.2018.01.008 | - |
dc.identifier.scopusid | 2-s2.0-85044859723 | - |
dc.identifier.wosid | 000435452300009 | - |
dc.identifier.bibliographicCitation | Molecular Therapy - Methods and Clinical Development, v.9, pp 90 - 98 | - |
dc.citation.title | Molecular Therapy - Methods and Clinical Development | - |
dc.citation.volume | 9 | - |
dc.citation.startPage | 90 | - |
dc.citation.endPage | 98 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | LEBERS CONGENITAL AMAUROSIS | - |
dc.subject.keywordPlus | RANDOMIZED CLINICAL-TRIAL | - |
dc.subject.keywordPlus | PIGMENT EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | MACULAR DEGENERATION | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | RETINAL TRANSDUCTION | - |
dc.subject.keywordPlus | DIABETIC-RETINOPATHY | - |
dc.subject.keywordPlus | INCREASED EXPRESSION | - |
dc.subject.keywordPlus | BIPOLAR CELLS | - |
dc.subject.keywordPlus | RECEPTOR 1 | - |
dc.subject.keywordAuthor | 의약학 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.