Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves' orbitopathy
DC Field | Value | Language |
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dc.contributor.author | Jang, Sun Young | - |
dc.contributor.author | Park, Seong Jun | - |
dc.contributor.author | Chae, Min Kyung | - |
dc.contributor.author | Lee, Joon H. | - |
dc.contributor.author | Lee, Eun Jig | - |
dc.contributor.author | Yoon, Jin Sook | - |
dc.date.accessioned | 2021-08-11T12:43:33Z | - |
dc.date.available | 2021-08-11T12:43:33Z | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 0007-1161 | - |
dc.identifier.issn | 1468-2079 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6167 | - |
dc.description.abstract | Aim To examine the role of microRNA-146a (miR-146a) in the regulation of fibrosis in an in vitro model of Graves' orbitopathy (GO). Methods Orbital fat/connective tissues were harvested from patients with GO and non-GO for primary orbital fibroblast cultures. The effects of transforming growth factor- (TGF-), a potent cytokine that promotes fibrosis, on miR-146a expression were analysed in GO and non-GO orbital fibroblasts using quantitative real-time PCR. The effects of overexpressed miR-146a on TGF--induced fibrotic markers were examined in GO orbital fibroblasts by western blot analysis. Expression ofSma and Mad related family (Smad) 4/tumour necrosis factor receptor-associated factor 6 (TRAF6) after transfection of miR-146a mimics or inhibitors were examined. Results TGF- induced an increase in miR-146a expression in orbital fibroblasts from patients with GO in a time-dependent and concentration-dependent manner. miR-146a mimics further decreased the production of TGF--induced fibronectin, collagen I and -smooth muscle actin protein. The Smad4 and TRAF6 protein levels were significantly decreased by miR-146a mimics, compared with control mimics, and significantly increased on inhibition of miR-146a production compared with a control. Conclusions miR-146a plays a role as a negative regulator in the production of TGF--induced fibrotic markers. Thus, miR-146a may be involved in the regulation of fibrosis in orbital fibroblasts from patients with GO. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BMJ Publishing Group | - |
dc.title | Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves' orbitopathy | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1136/bjophthalmol-2017-310723 | - |
dc.identifier.scopusid | 2-s2.0-85042873443 | - |
dc.identifier.wosid | 000429817700022 | - |
dc.identifier.bibliographicCitation | British Journal of Ophthalmology, v.102, no.3, pp 407 - 414 | - |
dc.citation.title | British Journal of Ophthalmology | - |
dc.citation.volume | 102 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 407 | - |
dc.citation.endPage | 414 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | SIGNALING PROTEINS | - |
dc.subject.keywordPlus | FIBROTIC RESPONSE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | CANCER CELL | - |
dc.subject.keywordPlus | OPHTHALMOPATHY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | experimental laboratory | - |
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