Proteomic identification of moesin upon exposure to acrolein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Pureun-Haneul | - |
dc.contributor.author | Kim, Byeong-Gon | - |
dc.contributor.author | Lee, Sun-Hye | - |
dc.contributor.author | Leikauf, George D. | - |
dc.contributor.author | Jang, An-Soo | - |
dc.date.accessioned | 2021-08-11T12:43:51Z | - |
dc.date.available | 2021-08-11T12:43:51Z | - |
dc.date.issued | 2018-01-17 | - |
dc.identifier.issn | 1477-5956 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6266 | - |
dc.description.abstract | Background: Acrolein (allyl Aldehyde) as one of smoke irritant exacerbates chronic airway diseases and increased in sputum of patients with asthma and chronic obstructive lung disease. But underlying mechanism remains unresolved. The aim of study was to identify protein expression in human lung microvascular endothelial cells (HMVEC-L) exposed to acrolein. Methods: A proteomic approach was used to determine the different expression of proteins at 8 h and 24 h after treatment of acrolein 30 nM and 300 nM to HMVEC-L. Treatment of HMVEC-L with acrolein 30 nM and 300 nM altered 21 protein spots on the two-dimensional gel, and these were then analyzed by MALDI-TOF MS. Results: These proteins included antioxidant, signal transduction, cytoskeleton, protein transduction, catalytic reduction. The proteins were classified into four groups according to the time course of their expression patterns such as continually increasing, transient increasing, transient decreasing, and continually decreasing. For validation immunohistochemical staining and Western blotting was performed on lung tissues from acrolein exposed mice. Moesin was expressed in endothelium, epithelium, and inflammatory cells and increased in lung tissues of acrolein exposed mice compared with sham treated mice. Conclusions: These results indicate that some of proteins may be an important role for airway disease exacerbation caused by acrolein exposure. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central | - |
dc.title | Proteomic identification of moesin upon exposure to acrolein | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/s12953-017-0130-4 | - |
dc.identifier.scopusid | 2-s2.0-85040732004 | - |
dc.identifier.wosid | 000422831000001 | - |
dc.identifier.bibliographicCitation | Proteome Science, v.16 | - |
dc.citation.title | Proteome Science | - |
dc.citation.volume | 16 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.subject.keywordPlus | OBSTRUCTIVE PULMONARY-DISEASE | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | GROWTH-FACTORS | - |
dc.subject.keywordPlus | TOBACCO-SMOKE | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | Acrolein | - |
dc.subject.keywordAuthor | Asthma | - |
dc.subject.keywordAuthor | HMVEC-L | - |
dc.subject.keywordAuthor | Moesin | - |
dc.subject.keywordAuthor | Proteomics | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.