두타산입술대고둥아재비 (Mirus junensis) 의 arginine kinase 유전자를 이용한 분자계통분류학적 연구

Abstract

Arginine kinase gene belongs to phosphagen super family and is one of the transferases such as glycocyamine kinase (GK), lombricine kinase (LK), taurocyamine kinase (TK) and creatine kinase (CK) which are expressed in inverterbrates. Arginine kinase (ArK) is commonly called arginine phosphokinase, adenosine 5'-triphosphate, L-arginine, N-phosphotransferase and plays an important role in transporting intracellular energy and regulating phosphoric acid concentration. ArK is also known to play a critical role in arginine and proline metabolism. In this study, we conducted transcriptomic analysis of Mirus junensis using the Next Generation Sequencer and all the sequences containing arginine kinase were obtained by using BLASTx against NCBI NR database and PANM database. We confirmed 358 amino acid residues and 1,074 bp coding region of ArK gene. Using BLAST analysis, we collected 14 similar sequences of M. junensis ArK. Analsysis of dendrogram derived from multiple sequence alignment using clustalW engine of MEGA 7 program showed that it is most similar to Nesiohelix samarangae and belongs to Mollusk with Aplysia kurodai, Conus ebraeus. The ArK of M. junensis needs further investigation about immune system and physiological study.