mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets
DC Field | Value | Language |
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dc.contributor.author | Choi, Cheolwon | - |
dc.contributor.author | Yoon, Seulgi | - |
dc.contributor.author | Moon, Hyesu | - |
dc.contributor.author | Bae, Yun-Ui | - |
dc.contributor.author | Kim, Chae-Bin | - |
dc.contributor.author | Diskul-Na-Ayudthaya, Penchatr | - |
dc.contributor.author | Trinh Van Ngu | - |
dc.contributor.author | Munir, Javaria | - |
dc.contributor.author | Han, JaeWook | - |
dc.contributor.author | Park, Se Bin | - |
dc.contributor.author | Moon, Jong-Seok | - |
dc.contributor.author | Song, Sujung | - |
dc.contributor.author | Ryu, Seongho | - |
dc.date.accessioned | 2021-08-11T13:43:55Z | - |
dc.date.available | 2021-08-11T13:43:55Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1547-6286 | - |
dc.identifier.issn | 1555-8584 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6820 | - |
dc.description.abstract | Techniques to isolate the small RNA fraction (<200nt) by column-based methods are commercially available. However, their use is limited because of the relatively high cost. We found that large RNA molecules, including mRNAs and rRNAs, are aggregated together in the presence of salts when RNA pellets are over-dried. Moreover, once RNA pellets are over-dried, large RNA molecules are barely soluble again during the elution process, whereas small RNA molecules (<100nt) can be eluted. We therefore modified the acid guanidinium thiocyanate-phenol-chloroform (AGPC)-based RNA extraction protocol by skipping the 70% ethanol washing step and over-drying the RNA pellet for 1hour at room temperature. We named this novel small RNA isolation method mirRICH. The quality of the small RNA sequences was validated by electrophoresis, next-generation sequencing, and quantitative PCR, and the findings support that our newly developed column-free method can successfully and efficiently isolate small RNAs from over-dried RNA pellets. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Landes Bioscience | - |
dc.title | mirRICH, a simple method to enrich the small RNA fraction from over-dried RNA pellets | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1080/15476286.2018.1451723 | - |
dc.identifier.scopusid | 2-s2.0-85045207455 | - |
dc.identifier.wosid | 000444092300011 | - |
dc.identifier.bibliographicCitation | RNA Biology, v.15, no.6, pp 763 - 772 | - |
dc.citation.title | RNA Biology | - |
dc.citation.volume | 15 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 763 | - |
dc.citation.endPage | 772 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | PHENOL-CHLOROFORM EXTRACTION | - |
dc.subject.keywordPlus | SOLID-PHASE EXTRACTION | - |
dc.subject.keywordPlus | SINGLE-STEP METHOD | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | SORBENTS | - |
dc.subject.keywordPlus | PROMOTES | - |
dc.subject.keywordPlus | DATABASE | - |
dc.subject.keywordAuthor | AGPC method | - |
dc.subject.keywordAuthor | small RNA enrichment | - |
dc.subject.keywordAuthor | quick method | - |
dc.subject.keywordAuthor | miRNA | - |
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