The Inactivation of ERK1/2, p38 and NF-kappa B Is Involved in the Down-Regulation of Osteoclastogenesis and Function by A2B Adenosine Receptor Stimulation
DC Field | Value | Language |
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dc.contributor.author | Kim, Bo Hyun | - |
dc.contributor.author | Oh, Ju Hee | - |
dc.contributor.author | Lee, Na Kyung | - |
dc.date.accessioned | 2021-08-11T14:24:13Z | - |
dc.date.available | 2021-08-11T14:24:13Z | - |
dc.date.issued | 2017-10 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7147 | - |
dc.description.abstract | A2B adenosine receptor (A2BAR) is known to be the regulator of bone homeostasis, but its regulatory mechanisms in osteoclast formation are less well-defined. Here, we demonstrate the effect of A2BAR stimulation on osteoclast differentiation and activity by RANKL. A2BAR was expressed in bone marrow-derived monocyte/macrophage (BMM) and RANKL increased A2BAR expression during osteoclastogenesis. A2BAR stimulation with its specific agonist BAY 60-6583 was sufficient to inhibit the activation of ERK1/2, p38 MAP kinases and NF-kappa B by RANKL as well as it abrogated cell-cell fusion in the late stage of osteoclast differentiation. Stimulation of A2BAR suppressed the expression of osteoclast marker genes, such as c-Fos, TRAP, Cathepsin-K and NFATc1, induced by RANKL, and transcriptional activity of NFATc1 was also inhibited by stimulation of A2BAR. A2BAR stimulation caused a notable reduction in the expression of Atp6v0d2 and DC-STAMP related to cell-cell fusion of osteoclasts. Especially, a decrease in bone resorption activity through suppression of actin ring formation by A2BAR stimulation was observed. Taken together, these results suggest that A2BAR stimulation inhibits the activation of ERK1/2, p38 and NF-kappa B by RANKL, which suppresses the induction of osteoclast marker genes, thus contributing to the decrease in osteoclast cell-cell fusion and bone resorption activity. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국분자세포생물학회 | - |
dc.title | The Inactivation of ERK1/2, p38 and NF-kappa B Is Involved in the Down-Regulation of Osteoclastogenesis and Function by A2B Adenosine Receptor Stimulation | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.14348/molcells.2017.0098 | - |
dc.identifier.scopusid | 2-s2.0-85049550242 | - |
dc.identifier.wosid | 000416400200006 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, v.40, no.10, pp 752 - 760 | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.volume | 40 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 752 | - |
dc.citation.endPage | 760 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002282861 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | CELL-CELL FUSION | - |
dc.subject.keywordPlus | BONE-RESORPTION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | A(1) | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | OSTEOPROTEGERIN | - |
dc.subject.keywordPlus | HOMEOSTASIS | - |
dc.subject.keywordPlus | LIGAND | - |
dc.subject.keywordAuthor | A2B adenosine receptor | - |
dc.subject.keywordAuthor | osteoclastogenesis | - |
dc.subject.keywordAuthor | RANKL | - |
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