BNIP3 induction by hypoxia stimulates FASN-dependent free fatty acid production enhancing therapeutic potential of umbilical cord blood-derived human mesenchymal stem cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun Jik | - |
dc.contributor.author | Jung, Young Hyun | - |
dc.contributor.author | Choi, Gee Euhn | - |
dc.contributor.author | Ko, So Hee | - |
dc.contributor.author | Lee, Sei-Jung | - |
dc.contributor.author | Lee, Sang Hun | - |
dc.contributor.author | Han, Ho Jae | - |
dc.date.accessioned | 2021-08-11T14:24:18Z | - |
dc.date.available | 2021-08-11T14:24:18Z | - |
dc.date.issued | 2017-10 | - |
dc.identifier.issn | 2213-2317 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7173 | - |
dc.description.abstract | Mitophagy under hypoxia is an important factor for maintaining and regulating stem cell functions. We previously demonstrated that fatty acid synthase (FASN) induced by hypoxia is a critical lipid metabolic factor determining the therapeutic efficacy of umbilical cord blood-derived human mesenchymal stem cells (UCB-hMSCs). Therefore, we investigated the mechanism of a major mitophagy regulator controlling lipid metabolism and therapeutic potential of UCB-hMSCs. This study revealed that Bcl2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3)-dependent mitophagy is important for reducing mitochondrial reactive oxygen species accumulation, anti-apoptosis, and migration under hypoxia. And, BNIP3 expression was regulated by CREB binding protein-mediated transcriptional actions of HIF-1 alpha and FOXO3. Silencing of BNIP3 suppressed free fatty acid (FFA) synthesis regulated by SREBP1/FASN pathway, which is involved in UCB-hMSC apoptosis via caspases cleavage and migration via cofilin-l-mediated F-actin reorganization in hypoxia. Moreover, reduced mouse skin wound-healing capacity of UCB-hMSC with hypoxia pretreatment by BNIP3 silencing was recovered by palmitic acid. Collectively, our findings suggest that BNIP3-mediated mitophagy under hypoxia leads to FASN-induced FFA synthesis, which is critical for therapeutic potential of UCB-hMSCs with hypoxia pretreatment. | - |
dc.format.extent | 18 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | BNIP3 induction by hypoxia stimulates FASN-dependent free fatty acid production enhancing therapeutic potential of umbilical cord blood-derived human mesenchymal stem cells | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.redox.2017.07.004 | - |
dc.identifier.scopusid | 2-s2.0-85022085396 | - |
dc.identifier.wosid | 000410470000038 | - |
dc.identifier.bibliographicCitation | Redox Biology, v.13, pp 426 - 443 | - |
dc.citation.title | Redox Biology | - |
dc.citation.volume | 13 | - |
dc.citation.startPage | 426 | - |
dc.citation.endPage | 443 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | TRIGLYCERIDE ACCUMULATION | - |
dc.subject.keywordPlus | INDUCIBLE FACTOR-1-ALPHA | - |
dc.subject.keywordPlus | LIPID-METABOLISM | - |
dc.subject.keywordPlus | TISSUE-REPAIR | - |
dc.subject.keywordPlus | MITOPHAGY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordAuthor | Hypoxia | - |
dc.subject.keywordAuthor | Bcl2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) | - |
dc.subject.keywordAuthor | Mitophagy | - |
dc.subject.keywordAuthor | Fatty acid synthase (FASN) | - |
dc.subject.keywordAuthor | Mesenchymal stem cell | - |
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