TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor
DC Field | Value | Language |
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dc.contributor.author | Kim, Soo Gon | - |
dc.contributor.author | Jo, Yong Hun | - |
dc.contributor.author | Seong, Jeong Hwan | - |
dc.contributor.author | Park, Ki Beom | - |
dc.contributor.author | Noh, Mi Young | - |
dc.contributor.author | Cho, Jun Ho | - |
dc.contributor.author | Ko, Hye Jin | - |
dc.contributor.author | Kim, Chang Eun | - |
dc.contributor.author | Tindwa, Hamisi | - |
dc.contributor.author | Patnaik, Bharat Bhusan | - |
dc.contributor.author | Bang, In Seok | - |
dc.contributor.author | Lee, Yong Seok | - |
dc.contributor.author | Han, Yeon Soo | - |
dc.date.accessioned | 2021-08-11T14:24:20Z | - |
dc.date.available | 2021-08-11T14:24:20Z | - |
dc.date.issued | 2017-10 | - |
dc.identifier.issn | 0965-1748 | - |
dc.identifier.issn | 1879-0240 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7182 | - |
dc.description.abstract | Scavenger receptors (SRs) constitute a family of membrane-bound receptors that bind to multiple ligands. The SR family of proteins is involved in removing cellular debris, oxidized low-density lipoproteins, and pathogens. Specifically, class C scavenger receptors (SR-C) have also been reported to be involved in phagocytosis of gram-positive and-negative bacteria in Drosophila and viruses in shrimp. However, reports are unavailable regarding the role of SR-C in antifungal immune mechanisms in insects. In this study, a full-length Tenebrio molitor SR-C (TmSR-C) sequence was obtained by 5'- and 3'-Rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The TmSR-C full-length cDNA comprised 1671 bp with 5'- and 3'-untranslated regions of 23 and 107-bp, respectively. TmSR-C encodes a putative protein of 556 amino acid residues that is constitutively expressed in all tissues of late instar larvae and 2-day-old adults, with the highest transcript levels observed in hemocytes of larvae and adults. TmSR-C mRNA showed a 2.5-fold and 3-fold increase at 24 and 6 h after infection with Candida albicans and D-glucan, respectively. Immunoassay with TmSR-C polyclonal antibody showed induction of the putative protein in the cytosols of hemocytes at 3 h after inoculation of C albicans. RNA interference (RNAi)-based gene silencing and phagocytosis assays were used to understand the role of TmSR-C in antifungal immunity. Silencing of TmSR-C transcripts reduced the survivability of late instar larvae at 2 days post-inoculation of C albicans, Escherichia coli, or Staphylococcus aureus. Furthermore, in TmSR-Csilenced larvae, there was a decline in the rate of microorganism phagocytosis. Taken together, results of this study suggest that TmSR-C plays a pivotal role in phagocytosing not only fungi but also gram negative and-positive bacteria in T. molitor. (C) 2017 Published by Elsevier Ltd. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Pergamon Press Ltd. | - |
dc.title | TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.ibmb.2017.08.007 | - |
dc.identifier.scopusid | 2-s2.0-85028715974 | - |
dc.identifier.wosid | 000413177300004 | - |
dc.identifier.bibliographicCitation | Insect Biochemistry and Molecular Biology, v.89, pp 31 - 42 | - |
dc.citation.title | Insect Biochemistry and Molecular Biology | - |
dc.citation.volume | 89 | - |
dc.citation.startPage | 31 | - |
dc.citation.endPage | 42 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Entomology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Entomology | - |
dc.subject.keywordPlus | PATTERN-RECOGNITION RECEPTOR | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | REGULATING EXPRESSION | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | CLASS-B | - |
dc.subject.keywordPlus | PHAGOCYTOSIS | - |
dc.subject.keywordPlus | PEPTIDOGLYCAN | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | MELANIZATION | - |
dc.subject.keywordPlus | INTERPROSCAN | - |
dc.subject.keywordAuthor | Tenebrio molitor | - |
dc.subject.keywordAuthor | Scavenger receptor class C | - |
dc.subject.keywordAuthor | Phagocytosis | - |
dc.subject.keywordAuthor | Innate immunity | - |
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