Effects of 1alpha, 25-dihydroxyvitamin D-3 on programmed cell death of Ishikawa endometrial cancer cells through ezrin phosphorylation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Tae-Hee | - |
dc.contributor.author | Park, Junsik | - |
dc.contributor.author | Lee, Jeong-Sang | - |
dc.contributor.author | Lee, Hae-Hyeog | - |
dc.date.accessioned | 2021-08-11T15:23:52Z | - |
dc.date.available | 2021-08-11T15:23:52Z | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 0144-3615 | - |
dc.identifier.issn | 1364-6893 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7619 | - |
dc.description.abstract | This study investigated the effects of 1, 25-dihydroxyvitamin D-3-induced cell death and its underlying molecular mechanisms in Ishikawa endometrial carcinoma cells. The effects of 1, 25-dihydroxyvitamin D-3 on Ishikawa cells were examined by 3-[4,5-dimethylthiazol-2-yl]-2.5-diphenyl-tetrazolium bromide, thiazolyl blue (MTT) assay. 1, 25-dihydroxyvitamin D-3 was shown to induce programmed cell death in Ishikawa endometrial carcinoma cells by activation of caspase-3 and caspase-9, along with elevation of Bcl-2 and Bcl-xL. Cell viability was reduced by 1, 25-dihydroxyvitamin D-3 in a concentration-dependent manner up to 2.5M. In addition, ezrin phosphorylation increased with the 1, 25-dihydroxyvitamin D-3 concentration (0-0.5M). The protein level of caspase-9 was increased by 1, 25-dihydroxyvitamin D-3 up to 0.5M. This is the first report regarding the efficacy and molecular mechanisms underlying the effects of 1, 25-dihydroxyvitamin D-3 in endometrial cancer cells. Our findings indicate that 1, 25-dihydroxyvitamin D-3 induces endometrial cancer cell death in a concentration-dependent manner.Impact statementUp to date, there is no report about the efficacy and molecular underlying mechanisms on the effect of vitamin D-3 in endometrial cancer cells. Our findings indicate that 1, 25-dihydroxyvitamin D-3. which is an active metabolite of vitamin D-3, induces Ishikawa endometrial cancer cell death in a concentration-dependent manner by activation of caspase-3 and -9, along with elevation of Bcl-2 and Bcl-xL. In addition, the same concentration of 1, 25-dihydroxyvitamin D-3 that provoked apoptotic signals caused phosphorylation of ezrin at threonine 567 in a VDR-dependent manner. This study suggests that 1, 25-dihydroxyvitamin D-3 within the optimal range (0.5 uM) would induce apoptosis through Fas-ezrin-caspase-3, -8, -9 signalling axis which may be a critical cell death regulator in Ishikawa endometrial cancer cell. Further study will be more interesting to address molecular connections or prove this critical optimal concentration range of vitamin D. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Taylor & Francis | - |
dc.title | Effects of 1alpha, 25-dihydroxyvitamin D-3 on programmed cell death of Ishikawa endometrial cancer cells through ezrin phosphorylation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1080/01443615.2016.1271777 | - |
dc.identifier.scopusid | 2-s2.0-85013792333 | - |
dc.identifier.wosid | 000399806600018 | - |
dc.identifier.bibliographicCitation | Journal of Obstetrics and Gynaecology, v.37, no.4, pp 503 - 509 | - |
dc.citation.title | Journal of Obstetrics and Gynaecology | - |
dc.citation.volume | 37 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 503 | - |
dc.citation.endPage | 509 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Obstetrics & Gynecology | - |
dc.relation.journalWebOfScienceCategory | Obstetrics & Gynecology | - |
dc.subject.keywordPlus | VITAMIN-D | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | ERM PROTEINS | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | FERM DOMAIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | calcitriol | - |
dc.subject.keywordAuthor | endometrial neoplasms | - |
dc.subject.keywordAuthor | membrane proteins | - |
dc.subject.keywordAuthor | vitamin D | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.