Effects of exogenous recombinant human bone morphogenic protein-7 on the corneal epithelial mesenchymal transition and fibrosis
DC Field | Value | Language |
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dc.contributor.author | Chung, Jin Kwon | - |
dc.contributor.author | Park, Shin Ae | - |
dc.contributor.author | Hwang, Hee Sun | - |
dc.contributor.author | Kim, Kwang Sung | - |
dc.contributor.author | Cho, Yang Je | - |
dc.contributor.author | You, Yong Sung | - |
dc.contributor.author | Kim, Young Sik | - |
dc.contributor.author | Jang, Ju Woong | - |
dc.contributor.author | Lee, Sung Jin | - |
dc.date.accessioned | 2021-08-11T15:24:06Z | - |
dc.date.available | 2021-08-11T15:24:06Z | - |
dc.date.issued | 2017-03-18 | - |
dc.identifier.issn | 2222-3959 | - |
dc.identifier.issn | 2227-4898 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7692 | - |
dc.description.abstract | AIM: To evaluate the effect of exogenous recombinant human bone morphogenic protein-7 (rhBMP-7) on transforming growth factor-alpha (TGF-beta)-induced epithelial mesenchymal cell transition (EMT) and assessed its antifibrotic effect via topical application. METHODS: The cytotoxic effect of rhBMP-7 was evaluated and the EMT of human corneal epithelial cells (HECEs) was induced by TGF-beta. HECEs were then cultured in the presence of rhBMP-7 and/or hyaluronic acid (HA). EMT markers, fibronectin, E-cadherin, alpha-smooth muscle actin (alpha-SMA), and matrix metaloproteinase-9 (MMP-9), were evaluated. The level of corneal fibrosis and the reepithelization rate were evaluated using a rabbit keratectomy model. Expression of alpha-SMA in keratocytes were quantified following treatment with different concentrations of rhBMP-7. RESULTS: Treatment with rhBMP-7 attenuated TGF-beta-induced EMT in HECEs. It significantly attenuated fibronectin secretion (31.6%; P<0.05), the alpha-SMA protein level (72.2%; P<0.01), and MMP-9 expression (23.6%, P<0.05) in HECEs compared with cells grown in the presence of TGF-6 alone. E-cadherin expression was significantly enhanced (289.7%; P<0.01) in the presence of rhBMP-7. Topical application of rhBMP-7 combined with 0.1% HA significantly reduced the amount of alpha-SMA(+) cells by 43.18% (P<0.05) at a concentration of 2.5 mu g/mL and by 47.73% (P<0.05) at 25 mu g/mL, compared with the control group, without disturbing corneal reepithelization. CONCLUSION: rhBMP-7 attenuates TGF-beta-induced EMT in vitro, and topical application of rhBMP-7 reduces keratocyte myodifferentiation during the early wound healing stages in vivo without hindering reepithelization. Topical rhBMP-7 application as biological eye drops seems to be feasible in diseases involving TGF-beta-related comeal fibrosis with corneal reepithelization disorders. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Effects of exogenous recombinant human bone morphogenic protein-7 on the corneal epithelial mesenchymal transition and fibrosis | - |
dc.type | Article | - |
dc.publisher.location | 중국 | - |
dc.identifier.doi | 10.18240/ijo.2017.03.01 | - |
dc.identifier.scopusid | 2-s2.0-85017091650 | - |
dc.identifier.wosid | 000396971200001 | - |
dc.identifier.bibliographicCitation | International Journal of Ophthalmology, v.10, no.3, pp 329 - 335 | - |
dc.citation.title | International Journal of Ophthalmology | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 329 | - |
dc.citation.endPage | 335 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | IN-SITU KERATOMILEUSIS | - |
dc.subject.keywordPlus | PHOTOREFRACTIVE KERATECTOMY | - |
dc.subject.keywordPlus | HYALURONIC-ACID | - |
dc.subject.keywordPlus | OCULAR SURFACE | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | bone morphogenic protein-7 | - |
dc.subject.keywordAuthor | corneal fibrosis | - |
dc.subject.keywordAuthor | epithelial mesenchymal transition | - |
dc.subject.keywordAuthor | myodifferentiation | - |
dc.subject.keywordAuthor | transforming growth factor-beta | - |
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