Surfactant 4-nonylphenyl-polyethylene glycol stimulates reactive oxygen species generation and apoptosis in human neuroblastoma cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zerin, Tanana | - |
dc.contributor.author | Song, Hoyeon | - |
dc.contributor.author | Gil, Hyowook | - |
dc.contributor.author | Hong, Saeyong | - |
dc.date.accessioned | 2021-08-11T15:24:08Z | - |
dc.date.available | 2021-08-11T15:24:08Z | - |
dc.date.issued | 2017-03-01 | - |
dc.identifier.issn | 1001-0742 | - |
dc.identifier.issn | 1878-7320 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7700 | - |
dc.description.abstract | The formulation of most pesticides is proprietary and individual components are therefore not generally known. In a preliminary study, we identified six compounds that are often present in pesticides, of which 4-nonylphenyl-polyethylene glycol (NP-40) was found to be the most toxic. In this study, we investigated the toxicity of NP-40 and underlying mechanism in neuronal SK-N-SH cells. Exposure to NP 40 at concentrations higher than 60 mu mol/L for 24 hr decreased cell viability. The cytotoxicity of NP-40 was time- and concentration-dependent. Nuclear fragmentation and chromatin condensation were apparent starting at 50 mu mol/L NP-40, and increased at higher concentrations. The expression of apoptotic factors including p53 and B-cell lymphoma (Bcl)-2-associated X protein was upregulated, while that of the anti-apoptotic marker Bcl-2 was downregulated at 80 mu mol/L NP-40. Cytochrome c release was observed from 80 to 100 mu mol/L by confocal microscopy. Caspase-9 and -3/7 activities increased according to concentration, and fluorescence-activated cell sorting analysis showed that apoptosis was induced at 50 mu mol/L and was increased at 80 mu mol/L. Our findings indicate that NP-40 stimulates the mitochondrial-mediated apoptosis pathway and reactive oxygen species production in a concentration-dependent manner, and suggest that antioxidant administration may be an effective treatment for patients with acute NP-40 poisoning. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B. V. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | IOS Press | - |
dc.title | Surfactant 4-nonylphenyl-polyethylene glycol stimulates reactive oxygen species generation and apoptosis in human neuroblastoma cells | - |
dc.type | Article | - |
dc.publisher.location | 중국 | - |
dc.identifier.doi | 10.1016/j.jes.2016.04.011 | - |
dc.identifier.scopusid | 2-s2.0-84969716713 | - |
dc.identifier.wosid | 000399326400027 | - |
dc.identifier.bibliographicCitation | Journal of Environmental Sciences, v.53, pp 262 - 268 | - |
dc.citation.title | Journal of Environmental Sciences | - |
dc.citation.volume | 53 | - |
dc.citation.startPage | 262 | - |
dc.citation.endPage | 268 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.subject.keywordPlus | GLYPHOSATE INTOXICATION | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordAuthor | Apoptosis Cytochrome c | - |
dc.subject.keywordAuthor | Caspase-3/7 | - |
dc.subject.keywordAuthor | Caspase-9 | - |
dc.subject.keywordAuthor | Nonionic surfactant | - |
dc.subject.keywordAuthor | Oxidative stress | - |
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