The effects of anti-vascular endothelial growth factor agents on human retinal pigment epithelial cells under high glucose conditions
DC Field | Value | Language |
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dc.contributor.author | Oh, Jong Rok | - |
dc.contributor.author | Han, Jung Woo | - |
dc.contributor.author | Kim, Yoon Kyung | - |
dc.contributor.author | Ohn, Young-Hoon | - |
dc.contributor.author | Park, Tae Kwann | - |
dc.date.accessioned | 2021-08-11T15:24:24Z | - |
dc.date.available | 2021-08-11T15:24:24Z | - |
dc.date.issued | 2017-02-18 | - |
dc.identifier.issn | 2222-3959 | - |
dc.identifier.issn | 2227-4898 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7782 | - |
dc.description.abstract | AIM: To investigate the effects of high glucose levels and anti-vascular endothelial growth factor (VEGF) agents (bevacizumab, ranibizumab and aflibercept) on retinal pigment epithelium (RPE) cells. METHODS: ARPE-19 cells were cultured at different glucose levels (5.5 mmol/L, 25 mmol/L, and 75 mmol/L). Cell viability was evaluated by MTT assay at 3d after treatment with D-glucose. Cell migration ability was measured by wound healing assay at 3d. A cell death detection kit was used to assess apoptosis at 3 and 14d. Cell proliferation was assessed by EdU assay at 3d. The culture medium was treated with anti-VEGF agents at clinically relevant concentrations. The experiment was then repeated at a different glucose level. RESULTS: The viability and migration of ARPE-19 cells were significantly decreased in the presence of 75 mmol/L as compared to 5.5 mmol/L glucose. The percentage of TUNEL-positive cells was significantly increased and the proliferative potential was decreased with 75 mmol/L compared to 5.5 mmol/L glucose. There were no significant differences in the results between 25 mmol/L and 5.5 mmol/L glucose. In the presence of 75 mmol/L glucose, the groups treated with anti-VEGF showed decreased cell viability and proliferation and increased apoptosis. However, there were no significant differences between the anti-VEGF groups. CONCLUSION: High glucose level decreases the viability, healing ability, and proliferation of RPE cells, while increasing apoptosis. Furthermore, anti-VEGF agents interfered with the physiological functions of RPE cells under high-glucose conditions, accompanied by decreases in cell viability and proliferation. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | The effects of anti-vascular endothelial growth factor agents on human retinal pigment epithelial cells under high glucose conditions | - |
dc.type | Article | - |
dc.publisher.location | 중국 | - |
dc.identifier.doi | 10.18240/ijo.2017.02.04 | - |
dc.identifier.scopusid | 2-s2.0-85014439124 | - |
dc.identifier.wosid | 000394478600004 | - |
dc.identifier.bibliographicCitation | International Journal of Ophthalmology, v.10, no.2, pp 203 - 210 | - |
dc.citation.title | International Journal of Ophthalmology | - |
dc.citation.volume | 10 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 203 | - |
dc.citation.endPage | 210 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordPlus | DIABETIC MACULAR EDEMA | - |
dc.subject.keywordPlus | VEGF-ANTAGONISTS | - |
dc.subject.keywordPlus | RANIBIZUMAB | - |
dc.subject.keywordPlus | BEVACIZUMAB | - |
dc.subject.keywordPlus | AFLIBERCEPT | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | PEGAPTANIB | - |
dc.subject.keywordAuthor | retinal pigment epithelium | - |
dc.subject.keywordAuthor | anti-vascular endothelial growth factor | - |
dc.subject.keywordAuthor | high glucose | - |
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