Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis
DC Field | Value | Language |
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dc.contributor.author | Lee, Jong-Uk | - |
dc.contributor.author | Cheong, Hyun Sub | - |
dc.contributor.author | Shim, Eun-Young | - |
dc.contributor.author | Bae, Da-Jeong | - |
dc.contributor.author | Chang, Hun Soo | - |
dc.contributor.author | Uh, Soo-Taek | - |
dc.contributor.author | Kim, Young Hoon | - |
dc.contributor.author | Park, Jong-Sook | - |
dc.contributor.author | Lee, Bora | - |
dc.contributor.author | Shin, Hyoung Doo | - |
dc.contributor.author | Park, Choon-Sik | - |
dc.date.accessioned | 2021-08-11T15:24:36Z | - |
dc.date.available | 2021-08-11T15:24:36Z | - |
dc.date.issued | 2017-01-05 | - |
dc.identifier.issn | 1465-9921 | - |
dc.identifier.issn | 1465-993X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7842 | - |
dc.description.abstract | Background: Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes. Methods: Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting > 2 absolute fold changes with p-values < 0.05. CCL8 mRNA and protein levels were quantified using real-time PCR and ELISA. CCL8 localization was evaluated by immunofluorescence staining. Results: One hundred seventy eight genes differentially expressed and 15 genes exhibited > 10-fold change. Among them, 13 were novel in relation with IPF. CCL8 expression was 22.8-fold higher in IPF fibroblasts. The levels of CCL8 mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (p = 0.022 and p = 0.026, respectively). The CCL8 concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (n = 22), hypersensitivity pneumonitis (n = 20) and sarcoidosis (n = 19) (p < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with CCL8 levels > 28.61 pg/mL showed shorter survival compared to those with lower levels (p = 0.012). CCL8 was expressed by a-SMA-positive cells in the interstitium of IPF. Conclusions: Transcriptome analysis identified several novel IPF-related genes. Among them, CCL8 is a candidate molecule for the differential diagnosis and prediction of survival. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central | - |
dc.title | Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/s12931-016-0493-6 | - |
dc.identifier.scopusid | 2-s2.0-85008214274 | - |
dc.identifier.wosid | 000391405300002 | - |
dc.identifier.bibliographicCitation | Respiratory Research, v.18 | - |
dc.citation.title | Respiratory Research | - |
dc.citation.volume | 18 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Respiratory System | - |
dc.relation.journalWebOfScienceCategory | Respiratory System | - |
dc.subject.keywordPlus | INTERSTITIAL PNEUMONIA | - |
dc.subject.keywordPlus | EXPRESSION PROFILES | - |
dc.subject.keywordPlus | CLASSIFICATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PROTEIN-1 | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | LUNGS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Gene expression | - |
dc.subject.keywordAuthor | IPF | - |
dc.subject.keywordAuthor | CCL8 | - |
dc.subject.keywordAuthor | Transcriptome | - |
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