멸종위기야생동물(I급) 귀이빨대칭이 (Cristaria plicata) 의 metallothionein 유전자를 활용한 분자계통분류학적 연구

Abstract

Cristaria plicata is enlisted as an endangered wild species class Ⅰ under the Red data list of National Institute of Biological Resources, South Korea. In this study, we have attempted to do molecular phylogenetic analysis by using metallothionein (CpMT) gene of C. plicata. The complete CpMT cDNA sequences were obtained from unigenes (analyzed NGS transcriptome data) of C. plicata. We used Illumina Hi-Seq 2500 next-generation sequencing platform to characterize the transcriptome resources of C. plicata. De novo analysis using the Trinity program and TGICL clustering resulted in the number of 374,794 (276,264,683 bp) unigenes. CpMT sequences were 210 bp coding for a protein 70 amino acid residues using sixpack (EMBOSS package) and genscan software. Multiple sequence alignment and phylogenetic analysis were performed by MEGA7 software. Molecular phylogenetic analysis indicates that CpMT has similarity with various mollusks such as Hyriopsis cumingii and Unio tumidus (Order Unionoida).