Netrin-1-Induced Stem Cell Bioactivity Contributes to the Regeneration of Injured Tissues via the Lipid Raft-Dependent Integrin alpha 6 beta 4 Signaling Pathway
DC Field | Value | Language |
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dc.contributor.author | Lee, Soo Sang | - |
dc.contributor.author | Lee, Sei-Jung | - |
dc.contributor.author | Lee, Sang Hun | - |
dc.contributor.author | Ryu, Jung Min | - |
dc.contributor.author | Lim, Hyeon Su | - |
dc.contributor.author | Kim, Jun Sung | - |
dc.contributor.author | Song, Eun Ju | - |
dc.contributor.author | Jung, Young Hyun | - |
dc.contributor.author | Lee, Hyun Jik | - |
dc.contributor.author | Kim, Chung Hun | - |
dc.contributor.author | Han, Ho Jae | - |
dc.date.accessioned | 2021-08-11T16:44:28Z | - |
dc.date.available | 2021-08-11T16:44:28Z | - |
dc.date.issued | 2016-11-24 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8597 | - |
dc.description.abstract | Netrin-1 (Ntn-1) is a multifunctional neuronal signaling molecule; however, its physiological significance, which improves the tissue-regeneration capacity of stem cells, has not been characterized. In the present study, we investigate the mechanism by which Ntn-1 promotes the proliferation of hUCB-MSCs with regard to the regeneration of injured tissues. We found that Ntn-1 induces the proliferation of hUCB-MSCs mainly via In alpha 6 beta 4 coupled with c-Src. Ntn-1 induced the recruitment of NADPH oxidases and Rac1 into membrane lipid rafts to facilitate ROS production. The In alpha 6 beta 4 signaling of Ntn-1 through ROS production is uniquely mediated by the activation of SP1 for cell cycle progression and the transcriptional occupancy of SP1 on the VEGF promoter. Moreover, Ntn-1 has the ability to induce the F-actin reorganization of hUCB-MSCs via the In alpha 6 beta 4 signaling pathway. In an in vivo model, transplantation of hUCB-MSCs pre-treated with Ntn-1 enhanced the skin wound healing process, where relatively more angiogenesis was detected. The potential effect of Ntn-1 on angiogenesis is further verified by the mouse hindlimb ischemia model, where the pre-activation of hUCB-MSCs with Ntn-1 significantly improved vascular regeneration. These results demonstrate that Ntn-1 plays an important role in the tissue regeneration process of hUCB-MSC via the lipid raft-mediated In alpha 6 beta 4 signaling pathway. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Netrin-1-Induced Stem Cell Bioactivity Contributes to the Regeneration of Injured Tissues via the Lipid Raft-Dependent Integrin alpha 6 beta 4 Signaling Pathway | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1038/srep37526 | - |
dc.identifier.scopusid | 2-s2.0-84997218064 | - |
dc.identifier.wosid | 000392062700001 | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.6 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 6 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | SP1 PHOSPHORYLATION | - |
dc.subject.keywordPlus | ARACHIDONIC-ACID | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | GUIDANCE | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | MOTILITY | - |
dc.subject.keywordAuthor | Netrin-1 | - |
dc.subject.keywordAuthor | Integrinα6β4 | - |
dc.subject.keywordAuthor | hUCB-MSCs | - |
dc.subject.keywordAuthor | Stem cell therapy | - |
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