Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression
DC Field | Value | Language |
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dc.contributor.author | Hwang, Sae Mi | - |
dc.contributor.author | Uhm, Tae Gi | - |
dc.contributor.author | Lee, Seol Kyung | - |
dc.contributor.author | Kong, Su-Kang | - |
dc.contributor.author | Jung, Kyung Hwa | - |
dc.contributor.author | Binas, Bert | - |
dc.contributor.author | Chai, Young Gyu | - |
dc.contributor.author | Park, Sung Woo | - |
dc.contributor.author | Chung, Il Yup | - |
dc.date.accessioned | 2021-08-11T16:46:28Z | - |
dc.date.available | 2021-08-11T16:46:28Z | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 0741-5400 | - |
dc.identifier.issn | 1938-3673 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8733 | - |
dc.description.abstract | Oligodendrocyte transcription factor 2, a basic helix-loop-helix transcription factor that binds to E-box motifs, is known to have a key role in determining lineage specification of oligodendrocytes and motor neurons. In the present study, we report that oligodendrocyte transcription factor 2 is expressed in human eosinophils and involved in transcriptional activation of the gene encoding sialic acid binding immunoglobulin-like lectin 8 (Siglec-8), a late eosinophil-differentiation marker known to exert eosinophil apoptosis. When cord blood CD34(+) hematopoietic stem cells differentiated toward eosinophils during a 24-d culture period, oligodendrocyte transcription factor 2 protein was expressed in cord blood eosinophils on d 24, a time when cord blood eosinophils are considered fully differentiated, whereas it was not detectable on d 18 or at earlier time points. Oligodendrocyte transcription factor 2 protein was also abundantly expressed in human peripheral-blood eosinophils but not in neutrophils, monocytes, lymphocytes, or cord blood mast cells. RNA sequencing analysis showed that numerous genes, especially those encoding eosinophil surface molecules, were highly up-regulated along with OLIG2. Among the genes examined, SIGLEC-8 messenger RNA and protein were markedly downregulated in parallel with OLIG2 by an oligodendrocyte transcription factor 2 small interfering RNA or a short hairpin RNA, as evidenced by real-time polymerase chain reaction, fluorescence-activated cell sorting, and Western blot analyses. In reporter gene and chromatin immunoprecipitation experiments, an E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind oligodendrocyte transcription factor 2. Hence, at least one important aspect of eosinophil differentiation is regulated by oligodendrocyte transcription factor 2, a transcription factor that has not previously been reported, to our knowledge, in normal granulocytes. | - |
dc.format.extent | 13 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Federation of American Societies for Experimental Biology | - |
dc.title | Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1189/jlb.1A0715-314RRR | - |
dc.identifier.scopusid | 2-s2.0-84990047796 | - |
dc.identifier.wosid | 000382849500010 | - |
dc.identifier.bibliographicCitation | Journal of Leukocyte Biology, v.100, no.4, pp 711 - 723 | - |
dc.citation.title | Journal of Leukocyte Biology | - |
dc.citation.volume | 100 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 711 | - |
dc.citation.endPage | 723 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | BHLH TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | MAJOR BASIC-PROTEIN | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MAST-CELLS | - |
dc.subject.keywordPlus | CCR3 TRANSCRIPTION | - |
dc.subject.keywordPlus | INTRONIC ENHANCER | - |
dc.subject.keywordPlus | NEUROTOXIN RNS2 | - |
dc.subject.keywordPlus | C/EBP-EPSILON | - |
dc.subject.keywordPlus | GATA FACTORS | - |
dc.subject.keywordPlus | RNA-SEQ | - |
dc.subject.keywordAuthor | cord blood | - |
dc.subject.keywordAuthor | eosinophil | - |
dc.subject.keywordAuthor | RNA-seq | - |
dc.subject.keywordAuthor | MBP | - |
dc.subject.keywordAuthor | MMP25 | - |
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