Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy
DC Field | Value | Language |
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dc.contributor.author | Jang, Sun Young | - |
dc.contributor.author | Chae, Min Kyung | - |
dc.contributor.author | Lee, Joon H. | - |
dc.contributor.author | Lee, Eun Jig | - |
dc.contributor.author | Yoon, Jin Sook | - |
dc.date.accessioned | 2021-08-11T17:24:50Z | - |
dc.date.available | 2021-08-11T17:24:50Z | - |
dc.date.issued | 2016-08 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.issn | 1552-5783 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8895 | - |
dc.description.abstract | PURPOSE. To investigate the role of microRNA 146a (miR-146a) in the regulation of inflammation in an in vitro model of Graves' orbitopathy (GO). METHODS. The level of miR-146a expression in orbital adipose tissue was compared between GO and non-GO by quantitative real-time PCR (qPCR). The effects of interleukin 1 beta (IL-1 beta) on miR-146a expression were analyzed in orbital fibroblasts by qPCR. To investigate the molecular mechanism underlying IL-1 beta-induced miR-146a expression, the effects of inhibitors of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B), mitogen-activated protein kinase/extracellular signal-regulated kinases (MEK)-1/2, c-Jun N-terminal kinases (JNK)-1/2, p38 MAP kinase, and phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) were analyzed. The effects of miR-146a mimics and inhibitors on IL-1 beta-induced IL-6 release were examined by ELISA and Western blotting. RESULTS. The level of miR-146a expression was significantly higher in GO orbital adipose tissue than in non-GO (P = 0.032). Interleukin 1 beta induced a time-and concentration-dependent increase in miR-146a expression. Interleukin 1 beta (10 ng/mL, 16 hours) induced an approximately 17.5-fold increase in miR-146 expression. The increase in miR-146a expression by IL-1 beta was significantly inhibited by NF-kappa B, JNK-1/2, and PI3K inhibitors (1.94 +/- 0.25, 5.28 +/- 0.34 and 9.73 +/- 2.32-fold, respectively, P < 0.05 compared with IL-1 beta-induced miR146 expression, independent t-test). Interleukin 1 beta-induced IL-6 protein production was further decreased by miR-146a mimics, but not by inhibitors of miR-146a. CONCLUSIONS. MicroRNA 146a was upregulated by inflammatory stress in orbital fibroblasts. Our results indicated that miR-146a had a positive effect on the anti-inflammatory process. MicroRNA 146a may play a role in the regulation of inflammation in orbital fibroblasts, and may participate in the pathogenesis of GO. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Association for Research in Vision and Ophthalmology | - |
dc.title | Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1167/iovs.16-19213 | - |
dc.identifier.scopusid | 2-s2.0-84983087306 | - |
dc.identifier.wosid | 000383981600010 | - |
dc.identifier.bibliographicCitation | Investigative Ophthalmology and Visual Science, v.57, no.10, pp 4027 - 4034 | - |
dc.citation.title | Investigative Ophthalmology and Visual Science | - |
dc.citation.volume | 57 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 4027 | - |
dc.citation.endPage | 4034 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordPlus | THYROID-ASSOCIATED OPHTHALMOPATHY | - |
dc.subject.keywordPlus | ORBITAL FIBROBLASTS | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | SIGNALING PROTEINS | - |
dc.subject.keywordPlus | RIBONUCLEIC-ACID | - |
dc.subject.keywordPlus | SYNOVIAL TISSUE | - |
dc.subject.keywordPlus | T-LYMPHOCYTES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MICRORNAS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | miR-146a | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | Graves' orbitopathy | - |
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