Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Min Jea | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Jo, Hyo Sang | - |
dc.contributor.author | Cho, Su Bin | - |
dc.contributor.author | Park, Jung Hwan | - |
dc.contributor.author | Lee, Chi Hern | - |
dc.contributor.author | Yeo, Eun Ji | - |
dc.contributor.author | Choi, Yeon Joo | - |
dc.contributor.author | Kim, Ji An | - |
dc.contributor.author | Hwang, Jung Soon | - |
dc.contributor.author | Sohn, Eun Jeong | - |
dc.contributor.author | Jeong, Ji-Heon | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Kwon, Hyeok Yil | - |
dc.contributor.author | Cho, Yong-Jun | - |
dc.contributor.author | Lee, Keunwook | - |
dc.contributor.author | Han, Kyu Hyung | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.accessioned | 2021-08-11T17:24:52Z | - |
dc.date.available | 2021-08-11T17:24:52Z | - |
dc.date.issued | 2016-08 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.issn | 1873-4596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8899 | - |
dc.description.abstract | Proline rich Akt substrate (PRAS40) is a component of mammalian target of rapamycin complex 1 (mTORC1) and is known to play an important role against reactive oxygen species-induced cell death. However, the precise function of PRAS40 in ischemia remains unclear. Thus, we investigated whether Tat-PRAS40, a cell-permeable fusion protein, has a protective function against oxidative stress-induced hippocampal neuronal (HT-22) cell death in an animal model of ischemia. We showed that Tat-PRAS40 transduced into HT-22 cells, and significantly protected against cell death by reducing the levels of H2O2 and derived reactive species, and DNA fragmentation as well as via the regulation of Bcl-2, Bax, and caspase 3 expression levels in H2O2 treated cells. Also, we showed that transduced Tat-PARS40 protein markedly increased phosphorylated RRAS40 expression levels and 14-3-3 sigma complex via the Akt signaling pathway. In an animal ischemia model, Tat-PRAS40 effectively transduced into the hippocampus in animal brain and significantly protected against neuronal cell death in the CAl region. We showed that Tat-PRAS40 protein effectively transduced into hippocampal neuronal cells and markedly protected against neuronal cell damage. Therefore, we suggest that Tat-PRAS40 protein may be used as a therapeutic protein for ischemia and oxidative stress-induced brain disorders. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 13 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2016.06.009 | - |
dc.identifier.scopusid | 2-s2.0-84976274771 | - |
dc.identifier.wosid | 000381924100023 | - |
dc.identifier.bibliographicCitation | Free Radical Biology and Medicine, v.97, pp 250 - 262 | - |
dc.citation.title | Free Radical Biology and Medicine | - |
dc.citation.volume | 97 | - |
dc.citation.startPage | 250 | - |
dc.citation.endPage | 262 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.subject.keywordPlus | RICH AKT-SUBSTRATE | - |
dc.subject.keywordPlus | INHIBITS INFLAMMATORY RESPONSE | - |
dc.subject.keywordPlus | PI3K/AKT SIGNALING PATHWAY | - |
dc.subject.keywordPlus | VIVO PROTEIN TRANSDUCTION | - |
dc.subject.keywordPlus | FOCAL CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | 40 KDA PRAS40 | - |
dc.subject.keywordPlus | OXYGEN RADICALS | - |
dc.subject.keywordPlus | NEURONAL DEATH | - |
dc.subject.keywordPlus | FUSION PROTEIN | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordAuthor | Brain ischemia | - |
dc.subject.keywordAuthor | Tat-PRAS40 | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | Cell death | - |
dc.subject.keywordAuthor | Protein therapy | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.