MiR-31/SDHA Axis Regulates Reprogramming Efficiency through Mitochondrial Metabolism
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Man Ryul | - |
dc.contributor.author | Mantel, Charlie | - |
dc.contributor.author | Lee, Sang A. | - |
dc.contributor.author | Moon, Sung-Hwan | - |
dc.contributor.author | Broxmeyer, Hal E. | - |
dc.date.accessioned | 2021-08-11T17:25:21Z | - |
dc.date.available | 2021-08-11T17:25:21Z | - |
dc.date.issued | 2016-07-12 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8937 | - |
dc.description.abstract | Metabolism is remodeled when somatic cells are reprogrammed into induced pluripotent stem cells (iPSCs), but the majority of iPSCs are not fully reprogrammed. In a shift essential for reprogramming, iPSCs use less mitochondrial respiration but increased anaerobic glycolysis for bioenergetics. We found that microRNA 31 (miR-31) suppressed succinate dehydrogenase complex subunit A (SDHA) expression, vital for mitochondrial electron transport chain (ETC) complex II. MiR-31 overexpression in partially reprogrammed iPSCs lowered SDHA expression levels and oxygen consumption rates to that of fully reprogrammed iPSCs, but did not increase the proportion of fully reprogrammed TRA1-60(+) cells in colonies unless miR-31 was co-transduced with Yamanaka factors, which resulted in a 2.7-fold increase in full reprogramming. Thus switching from mitochondrial respiration to glycolytic metabolism through regulation of the miR-31/SDHA axis is critical for lowering the reprogramming threshold. This is supportive of multi-stage reprogramming whereby metabolic remodeling is fundamental. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Cell Press | - |
dc.title | MiR-31/SDHA Axis Regulates Reprogramming Efficiency through Mitochondrial Metabolism | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.stemcr.2016.05.012 | - |
dc.identifier.scopusid | 2-s2.0-84992053039 | - |
dc.identifier.wosid | 000380492300001 | - |
dc.identifier.bibliographicCitation | Stem Cell Reports, v.7, no.1, pp 1 - 10 | - |
dc.citation.title | Stem Cell Reports | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | COMPLEX-II | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordAuthor | iPS | - |
dc.subject.keywordAuthor | metabolism | - |
dc.subject.keywordAuthor | reprogramming | - |
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