Tat-NOL3 protects against hippocampal neuronal cell death induced by oxidative stress through the regulation of apoptotic pathways
DC Field | Value | Language |
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dc.contributor.author | Sohn, Eun Jeong | - |
dc.contributor.author | Shin, Min Jea | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Yong, Ji In | - |
dc.contributor.author | Ryu, Eun Ji | - |
dc.contributor.author | Park, Jung Hwan | - |
dc.contributor.author | Bin Cho, Su | - |
dc.contributor.author | Cha, Hyun Ju | - |
dc.contributor.author | Kim, Sang Jin | - |
dc.contributor.author | Yeo, Hyeon Ji | - |
dc.contributor.author | Yeo, Eun Ji | - |
dc.contributor.author | Choi, Yeon Joo | - |
dc.contributor.author | Im, Seung Kwon | - |
dc.contributor.author | Kweon, Hae Young | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Yu, Yeon Hee | - |
dc.contributor.author | Cho, Sung-Woo | - |
dc.contributor.author | Park, Meeyoung | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Cho, Yong-Jun | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.accessioned | 2021-08-11T17:25:35Z | - |
dc.date.available | 2021-08-11T17:25:35Z | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.issn | 1791-244X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8969 | - |
dc.description.abstract | Oxidative stress-induced apoptosis is associated with neuronal cell death and ischemia. The NOL3 [nucleolar protein 3 (apoptosis repressor with CARD domain)] protein protects against oxidative stress-induced cell death. However, the protective mechanism responsible for this effect as well as the effects of NOL3 against oxidative stress in ischemia remain unclear. Thus, we examined the protective effects of NOL3 protein on hydrogen peroxide (H2O2)-induced oxidative stress and the mechanism responsible for these effects in hippocampal neuronal HT22 cells and in an animal model of forebrain ischemia using Tat-fused NOL3 protein (Tat-NOL3). Purified Tat-NOL3 protein transduced into the H2O2-exposed HT22 cells and inhibited the production of reactive oxygen species (ROS), DNA fragmentation and reduced mitochondrial membrane potential (Delta(Psi m)). In addition, Tat-NOL3 prevented neuronal cell death through the regulation of apoptotic signaling pathways including Bax, Bcl-2, caspase-2, -3 and -8, PARP and p53. In addition, Tat-NOL3 protein transduced into the animal brains and significantly protected against neuronal cell death in the CA1 region of the hippocampus by regulating the activation of microglia and astrocytes. Taken together, these findings demonstrate that Tat-NOL3 protein protects against oxidative stress-induced neuronal cell death by regulating oxidative stress and by acting as an anti-apoptotic protein. Thus, we suggest that Tat-NOL3 represents a potential therapeutic agent for protection against ischemic brain injury. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Demetrios A. Spandidos Ed. & Pub. | - |
dc.title | Tat-NOL3 protects against hippocampal neuronal cell death induced by oxidative stress through the regulation of apoptotic pathways | - |
dc.type | Article | - |
dc.publisher.location | 그리이스 | - |
dc.identifier.doi | 10.3892/ijmm.2016.2596 | - |
dc.identifier.scopusid | 2-s2.0-84978818665 | - |
dc.identifier.wosid | 000381289700025 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Medicine, v.38, no.1, pp 225 - 235 | - |
dc.citation.title | International Journal of Molecular Medicine | - |
dc.citation.volume | 38 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 225 | - |
dc.citation.endPage | 235 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | MITOCHONDRIAL PATHWAY | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | GLOBAL-ISCHEMIA | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | ischemic damage | - |
dc.subject.keywordAuthor | cell viability | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | Tat-fused NOL3 protein | - |
dc.subject.keywordAuthor | protein therapy | - |
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