Tat-ATOX1 inhibits streptozotocin-induced cell death in pancreatic RINm5F cells and attenuates diabetes in a mouse model
DC Field | Value | Language |
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dc.contributor.author | Ahn, Eun Hee | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Shin, Min Jea | - |
dc.contributor.author | Ryu, Eun Ji | - |
dc.contributor.author | Yong, Ji In | - |
dc.contributor.author | Chung, Seok Young | - |
dc.contributor.author | Cha, Hyun Ju | - |
dc.contributor.author | Kim, Sang Jin | - |
dc.contributor.author | Choi, Yeon Joo | - |
dc.contributor.author | Kim, Duk-Soo | - |
dc.contributor.author | Cho, Sung-Woo | - |
dc.contributor.author | Lee, Keunwook | - |
dc.contributor.author | Cho, Yoon Shin | - |
dc.contributor.author | Kwon, Hyeok Yil | - |
dc.contributor.author | Park, Jinseu | - |
dc.contributor.author | Eum, Won Sik | - |
dc.contributor.author | Choi, Soo Young | - |
dc.date.accessioned | 2021-08-11T17:25:37Z | - |
dc.date.available | 2021-08-11T17:25:37Z | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.issn | 1791-244X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8970 | - |
dc.description.abstract | Antioxidant 1 (ATOX1) functions as an antioxidant against hydrogen peroxide and superoxide, and therefore may play a significant role in many human diseases, including diabetes mellitus (DM). In the present study, we examined the protective effects of Tat-ATOX1 protein on streptozotocin (STZ)-exposed pancreatic insulinoma cells (RINm5F) and in a mouse model of STZ-induced diabetes using western blot analysis, immunofluorescence staining and MTT assay, as well as histological and biochemical analysis. Purified Tat-ATOX1 protein was efficiently transduced into RINm5F cells in a dose-and time-dependent manner. Additionally, Tat-ATOX1 protein markedly inhibited reactive oxygen species (ROS) production, DNA damage and the activation of Akt and mitogen activated protein kinases (MAPKs) in STZ-exposed RINm5F cells. In addition, Tat-ATOX1 protein transduced into mice pancreatic tissues and significantly decreased blood glucose and hemoglobin A1c (HbA1c) levels as well as the body weight changes in a model of STZ-induced diabetes. These results indicate that transduced Tat-ATOX1 protein protects pancreatic beta-cells by inhibiting STZ-induced cellular toxicity in vitro and in vivo. Based on these findings, we suggest that Tat-ATOX1 protein has potential applications as a therapeutic agent for oxidative stress-induced diseases including DM. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Demetrios A. Spandidos Ed. & Pub. | - |
dc.title | Tat-ATOX1 inhibits streptozotocin-induced cell death in pancreatic RINm5F cells and attenuates diabetes in a mouse model | - |
dc.type | Article | - |
dc.publisher.location | 그리이스 | - |
dc.identifier.doi | 10.3892/ijmm.2016.2599 | - |
dc.identifier.scopusid | 2-s2.0-84978909259 | - |
dc.identifier.wosid | 000381289700024 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Medicine, v.38, no.1, pp 217 - 224 | - |
dc.citation.title | International Journal of Molecular Medicine | - |
dc.citation.volume | 38 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 217 | - |
dc.citation.endPage | 224 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | HYPERGLYCEMIA | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordAuthor | Tat-antioxidant 1 | - |
dc.subject.keywordAuthor | diabetes mellitus | - |
dc.subject.keywordAuthor | blood glucose | - |
dc.subject.keywordAuthor | hemoglobin A1c | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | protein therapy | - |
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