Regulation of c-Myc Expression by Ahnak Promotes Induced Pluripotent Stem Cell Generation
DC Field | Value | Language |
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dc.contributor.author | Lim, Hee Jung | - |
dc.contributor.author | Kim, Jusong | - |
dc.contributor.author | Park, Chang-Hwan | - |
dc.contributor.author | Lee, Sang A. | - |
dc.contributor.author | Lee, Man Ryul | - |
dc.contributor.author | Kim, Kye-Seong | - |
dc.contributor.author | Kim, Jaesang | - |
dc.contributor.author | Bae, Yun Soo | - |
dc.date.accessioned | 2021-08-11T18:24:23Z | - |
dc.date.available | 2021-08-11T18:24:23Z | - |
dc.date.issued | 2016-01-08 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/9418 | - |
dc.description.abstract | We have previously reported that Ahnak-mediated TGF beta signaling leads to down-regulation of c-Myc expression. Here, we show that inhibition of Ahnak can promote generation of induced pluripotent stem cells (iPSC) via up-regulation of endogenous c-Myc. Consistent with the c-Myc inhibitory role of Ahnak, mouse embryonic fibroblasts from Ahnak-deficient mouse (Ahnak(-/-) MEF) show an increased level of c-Myc expression compared with wild type MEF. Generation of iPSC with just three of the four Yamanaka factors, Oct4, Sox2, and Klf4 (hereafter 3F), was significantly enhanced in Ahnak(-/-) MEF. Similar results were obtained when Ahnak-specific shRNA was applied to wild type MEF. Of note, expression of Ahnak was significantly induced during the formation of embryoid bodies from embryonic stem cells, suggesting that Ahnak-mediated c-Myc inhibition is involved in embryoid body formation and the initial differentiation of pluripotent stem cells. The iPSC from 3F-infected Ahnak(-/-) MEF cells (Ahnak(-/-) - iPSC-3F) showed expression of all stem cell markers examined and the capability to form three primary germ layers. Moreover, injection of Ahnak(-/-) - iPSC-3F into athymic nude mice led to development of teratoma containing tissues from all three primary germ layers, indicating that iPSC from Ahnak(-/-) MEF are bona fide pluripotent stem cells. Taken together, these data provide evidence for a new role for Ahnak in cell fate determination during development and suggest that manipulation of Ahnak and the associated signaling pathway may provide a means to regulate iPSC generation. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.title | Regulation of c-Myc Expression by Ahnak Promotes Induced Pluripotent Stem Cell Generation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1074/jbc.M115.659276 | - |
dc.identifier.scopusid | 2-s2.0-84954163900 | - |
dc.identifier.wosid | 000367830500023 | - |
dc.identifier.bibliographicCitation | Journal of Biological Chemistry, v.291, no.2, pp 752 - 761 | - |
dc.citation.title | Journal of Biological Chemistry | - |
dc.citation.volume | 291 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 752 | - |
dc.citation.endPage | 761 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | KRUPPEL-LIKE FACTORS | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | c-Myc | - |
dc.subject.keywordAuthor | Ahnak | - |
dc.subject.keywordAuthor | InducedPluripotent Stem Cell | - |
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