加減生血潤膚飮의 당뇨병 치료효과 확인을 위한 생리활성성분 분석과 경구포도당부하 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 인정도 | - |
dc.contributor.author | 임대식 | - |
dc.contributor.author | 김원일 | - |
dc.date.accessioned | 2021-08-11T18:44:51Z | - |
dc.date.available | 2021-08-11T18:44:51Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1229-1218 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/9812 | - |
dc.description.abstract | Objectives : Instrumental chemical analysis was utilized to investigate the effect of Add-Omit-Saenghyeoryunbu-eum(AO-SHU) on diabetic treatment. One of the most exciting, yet also controversial, arguments is the safety and biological mechanisms of the natural medicine on human body. Therefore, the aim of this study is to provide a better understanding on bioactive chemical components, hazards of heavy metal contamination and biological mechanism of the diabetic medicine composed of 12 different natural herbs. Methods : To study bioactive compound and metallic component in the diabetic medicine in detail, LC-MS/MS (Liquid Chromatography-Mass/Mass), GC (Gas Chromatography) and ICP (Inductively Coupled Plasma) were utilized to characterize the extract of the diabetic medicine and the result was compared with 18 marker substances selected from literature survey. In addition, in vitro assay experiments including GPR 119 activity and human DGAT-1 inhibition, and OGTT (Oral Glucose Tolerance Test) were performed to verify the effectiveness of this medicine on diabetic treatment. Results : Out of 18 marker substances, 9 bioactive compounds were identified from LC-MS/MS analysis which include Citruline, Catalpol, Berberine, Ginsenoside Rb1, Ginsenoside Rg1, Oleanolic acid, β-Sitosterol, Mangiferin, and Schizandrin. ICP study on 245 residual pesticides revealed that 239 species were not detected but 6 species, Dimethomorph, Trifloxystrobin, Pyraclostrobin, Isoprocarb, Carbaryl and Flubendiamide, while the amounts are trace levels, below permitted concentrations. The biological activity was observed in vitro assay and Oral Glucose Tolerance Test(OGTT), which are consistent with a preliminary clinical test result, a drop in blood sugar level after taking this herbal medicine. Conclusions : Instrumental chemical analysis using LC-MS/MS, GC, and ICP was conducted successfully to identify bioactive compounds in AO-SHU for the treatment of diabetes, finding 9 bioactive compounds. Furthermore, in vitro assay experiments and OGTT show that AO-SHU has its biological activities, which imply that it can be a candidate for the future diabetes remedy. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한한의학방제학회 | - |
dc.title | 加減生血潤膚飮의 당뇨병 치료효과 확인을 위한 생리활성성분 분석과 경구포도당부하 연구 | - |
dc.title.alternative | Studies about the bioactive component analysis and an oral glucose tolerance test of Add-Omit-Saenghyeoryunbu-eum(AO-SHU) for confirmation of diabetes therapy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 임대식 | - |
dc.identifier.doi | 10.14374/HFS.2016.24.2.80 | - |
dc.identifier.bibliographicCitation | 대한한의학 방제학회지, v.24, no.2, pp.80 - 99 | - |
dc.relation.isPartOf | 대한한의학 방제학회지 | - |
dc.citation.title | 대한한의학 방제학회지 | - |
dc.citation.volume | 24 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 80 | - |
dc.citation.endPage | 99 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART002112327 | - |
dc.description.journalClass | 2 | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Add-Omit-Saenghyeoryunbu-eum(AO-SHU) | - |
dc.subject.keywordAuthor | Diabetes Therapy | - |
dc.subject.keywordAuthor | Pharmacological active ingredient | - |
dc.subject.keywordAuthor | Instrumental analysis | - |
dc.subject.keywordAuthor | OGTT | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.