Evaluating the effects of honey bee (Apis mellifera L.) venom on the expression of insulin sensitivity and inflammation-related genes in co-culture of adipocytes and macrophages
DC Field | Value | Language |
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dc.contributor.author | Kim, HY[Kim, Hee-Yeon] | - |
dc.contributor.author | Jo, MJ[Jo, Min Jeong] | - |
dc.contributor.author | Nam, SY[Nam, So Yung] | - |
dc.contributor.author | Kim, KM[Kim, Kwang Min] | - |
dc.contributor.author | Choi, MB[Choi, Moon Bo] | - |
dc.contributor.author | Lee, YH[Lee, Yong-Ho] | - |
dc.date.accessioned | 2021-07-28T10:28:24Z | - |
dc.date.available | 2021-07-28T10:28:24Z | - |
dc.date.created | 2020-10-12 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 1738-2297 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/4708 | - |
dc.description.abstract | Obese adipose tissue is characterized by adipocyte hypertrophy and a massive macrophage infiltration. The interaction between macrophages with mature adipocytes releases pro-inflammatory cytokines. This chronic inflammatory state can contribute to obesity-related complications, such as insulin resistance, type 2 diabetes and cardiovascular disease. Therefore, we can attempt to prevent and treat obesity-related diseases by inhibiting macrophage infiltration and blocking their interaction with adipocytes. Honey bee (Apis mellifera) venom (BV) has been reported to have anti-inflammatory effects. Although BV is used to treat chronic inflammatory diseases, few studies have addressed its use in obesity-associated inflammation. This study examines the inhibitory effects of BV on lipid accumulation in differentiating preadipocytes, inflammation, and insulin resistance in macrophages and adipocyte-macrophage co-culture system. We treated 3 T3-L1 preadipocytes with BV during differentiation. We later measured lipid accumulation and gene expression of master adipogenic transcription factors. After RAW264.7 and 3 T3-L1 cells were pretreated with BV, RAW264.7 cells were activated with LPS or co-cultured with pretreated 3 T3-L1 cells. Gene expression of pro-inflammatory cytokines and insulin sensitizing genes was measured in these cells. BV inhibited lipid accumulation and C/EBP alpha and PPAR gamma gene expression during intermediate and late 3 T3-L1 cell differentiation. BV also suppressed gene expression of pro-inflammatory cytokines (COX-2, iNOS, MCP-1, TNF-alpha, IL-1 beta and IL-6) in LPS-stimulated macrophages, and in co-culture of 3 T3-L1 adipocytes and RAW264.7 macrophages. However, adiponectin and GLUT-4 expression were both significantly increased by BV in co-culture. These findings demonstrate that BV attenuates adipocyte hypertrophy and improves obesity-related inflammation and insulin resistance in obese adipose tissue. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | INHIBITION | - |
dc.subject | MELITTIN | - |
dc.subject | OBESITY | - |
dc.subject | DISEASE | - |
dc.subject | RESPONSES | - |
dc.title | Evaluating the effects of honey bee (Apis mellifera L.) venom on the expression of insulin sensitivity and inflammation-related genes in co-culture of adipocytes and macrophages | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, KM[Kim, Kwang Min] | - |
dc.identifier.doi | 10.1111/1748-5967.12431 | - |
dc.identifier.scopusid | 2-s2.0-85085495300 | - |
dc.identifier.wosid | 000536484700004 | - |
dc.identifier.bibliographicCitation | ENTOMOLOGICAL RESEARCH, v.50, no.5, pp.236 - 244 | - |
dc.relation.isPartOf | ENTOMOLOGICAL RESEARCH | - |
dc.citation.title | ENTOMOLOGICAL RESEARCH | - |
dc.citation.volume | 50 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 236 | - |
dc.citation.endPage | 244 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Entomology | - |
dc.relation.journalWebOfScienceCategory | Entomology | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MELITTIN | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordAuthor | adipocyte | - |
dc.subject.keywordAuthor | adipogenesis | - |
dc.subject.keywordAuthor | bee venom | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | macrophage | - |
dc.subject.keywordAuthor | obesity | - |
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