Schisandrin B suppresses TGF beta 1-induced stress fiber formation by inhibiting myosin light chain phosphorylation
DC Field | Value | Language |
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dc.contributor.author | Chun, JN[Chun, Jung Nyeo] | - |
dc.contributor.author | Kim, SY[Kim, Sang-Yeob] | - |
dc.contributor.author | Park, EJ[Park, Eun-Jung] | - |
dc.contributor.author | Kwon, EJ[Kwon, Eun Jung] | - |
dc.contributor.author | Bae, DJ[Bae, Dong-Jun] | - |
dc.contributor.author | Kim, IS[Kim, In-San] | - |
dc.contributor.author | Kim, HK[Kim, Hye Kyung] | - |
dc.contributor.author | Park, JK[Park, Jong Kwan] | - |
dc.contributor.author | Lee, SW[Lee, Sung Won] | - |
dc.contributor.author | Park, HH[Park, Hyun Ho] | - |
dc.contributor.author | So, I[So, Insuk] | - |
dc.contributor.author | Jeon, JH[Jeon, Ju-Hong] | - |
dc.date.accessioned | 2021-08-03T17:50:17Z | - |
dc.date.available | 2021-08-03T17:50:17Z | - |
dc.date.created | 2016-08-06 | - |
dc.date.issued | 2014-03-14 | - |
dc.identifier.issn | 0378-8741 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/53665 | - |
dc.description.abstract | Ethnopharmacological relevance: Schisandra chinensis fruit extract (SCE) has been used as a traditional oriental medicine for treating vascular diseases. However, the pharmacologic effects and mechanisms of SCE on vascular fibrosis are still largely unknown. Transforming growth factor beta 1 (TGF beta 1)-mediated cellular changes are closely associated with the pathogenesis of vascular fibrotic diseases. Particularly, TGF beta 1 induces actin stress fiber formation that is a crucial mechanism underlying vascular smooth muscle cell (VSMC) migration in response to vascular injury. In this study, we investigated the effect of SCE and its active ingredients on TGF beta 1-induced stress fiber assembly in A7r5 VSMCs. Materials and methods: To investigate pharmacological actions of SCE and its ingredients on TGF beta 1-treated VSMCs, we have employed molecular and cell biological technologies, such as confocal microscopy, fluorescence resonance energy transfer, western blotting, and radiometric enzyme analyses. Results: We found that SCE inhibited TGF beta 1-induced stress fiber formation and cell migration. Schisandrin B (SchB) showed the most prominent effect among the active ingredients of SCE tested. SchB reduced TGF beta 1-mediated phosphorylation of myosin light chain, and this effect was independent of RhoA/Rho-associated kinase pathway. Fluorescence resonance energy transfer and radiometric enzyme assays confirmed that SchB inhibited myosin light chain kinase activity. We also showed that SchB decreased TGF beta 1-mediated induction of alpha-smooth muscle actin by inhibiting Smad signaling. Conclusions: The present study demonstrates that SCE and its active ingredient SchB suppressed TGF beta 1-induced stress fiber formation at the molecular level. Therefore, our findings may help future investigations to develop multi-targeted therapeutic strategies that attenuate VSMC migration and vascular fibrosis. (C) 2014 Elsevier Ireland Ltd. All rights reserved. | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | VASCULAR SMOOTH-MUSCLE | - |
dc.subject | TGF-BETA | - |
dc.subject | CHINENSIS EXTRACT | - |
dc.subject | BARRIER FUNCTION | - |
dc.subject | RHO-KINASE | - |
dc.subject | CELLS | - |
dc.subject | EXPRESSION | - |
dc.subject | MIGRATION | - |
dc.subject | DISEASE | - |
dc.subject | PERMEABILITY | - |
dc.title | Schisandrin B suppresses TGF beta 1-induced stress fiber formation by inhibiting myosin light chain phosphorylation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, SW[Lee, Sung Won] | - |
dc.identifier.doi | 10.1016/j.jep.2014.01.024 | - |
dc.identifier.scopusid | 2-s2.0-84896713372 | - |
dc.identifier.wosid | 000333776700017 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ETHNOPHARMACOLOGY, v.152, no.2, pp.364 - 371 | - |
dc.relation.isPartOf | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.citation.title | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.citation.volume | 152 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 364 | - |
dc.citation.endPage | 371 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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