RNA interference-mediated simultaneous silencing of four genes using cross-shaped RNA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, TY[Lee, Tae Yeon] | - |
dc.contributor.author | Chang, CI[Chang, Chan Il] | - |
dc.contributor.author | Lee, D[Lee, Dooyoung] | - |
dc.contributor.author | Hong, SW[Hong, Sun Woo] | - |
dc.contributor.author | Shin, C[Shin, Chanseok] | - |
dc.contributor.author | Li, CJ[Li, Chiang J.] | - |
dc.contributor.author | Kim, S[Kim, Soyoun] | - |
dc.contributor.author | Haussecker, D[Haussecker, Dirk] | - |
dc.contributor.author | Lee, DK[Lee, Dong-ki] | - |
dc.date.accessioned | 2021-08-04T18:59:34Z | - |
dc.date.available | 2021-08-04T18:59:34Z | - |
dc.date.created | 2016-08-06 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/60981 | - |
dc.description.abstract | The structural flexibility of RNA interference (RNAi)-triggering nucleic acids suggests that the design of unconventional RNAi trigger structures with novel features is possible. Here, we report a cross-shaped RNA duplex structure, termed quadruple interfering RNA (qiRNA), with multiple target gene silencing activity. qiRNA triggers the simultaneous down-regulation of four cellular target genes via an RNAi mechanism. In addition, qiRNA shows enhanced intracellular delivery and target gene silencing over conventional siRNA when complexed with jetPEI, a linear polyethyleneimine (PEI). We also show that the long antisense strand of qiRNA is incorporated intact into an RNA-induced silencing complex (RISC). This novel RNA scaffold further expands the repertoire of RNAi-triggering molecular structures and could be used in the development of therapeutics for various diseases including viral infections and cancer. | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.subject | IN-VIVO | - |
dc.subject | BIOGENESIS | - |
dc.subject | ARGONAUTE2 | - |
dc.subject | PATHWAY | - |
dc.subject | SIRNAS | - |
dc.title | RNA interference-mediated simultaneous silencing of four genes using cross-shaped RNA | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, DK[Lee, Dong-ki] | - |
dc.identifier.doi | 10.1007/s10059-013-2316-7 | - |
dc.identifier.scopusid | 2-s2.0-84880699897 | - |
dc.identifier.wosid | 000318488700008 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.35, no.4, pp.320 - 326 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 35 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 320 | - |
dc.citation.endPage | 326 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | BIOGENESIS | - |
dc.subject.keywordPlus | ARGONAUTE2 | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | SIRNAS | - |
dc.subject.keywordAuthor | polyethyleneimine (PEI) | - |
dc.subject.keywordAuthor | quadruple interfering RNA | - |
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