O-GlcNAc Regulates Pluripotency and Reprogramming by Directly Acting on Core Components of the Pluripotency Network
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, H[Jang, Hyonchol] | - |
dc.contributor.author | Kim, TW[Kim, Tae Wan] | - |
dc.contributor.author | Yoon, S[Yoon, Sungho] | - |
dc.contributor.author | Choi, SY[Choi, Soo-Youn] | - |
dc.contributor.author | Kang, TW[Kang, Tae-Wook] | - |
dc.contributor.author | Kim, SY[Kim, Seon-Young] | - |
dc.contributor.author | Kwon, YW[Kwon, Yoo-Wook] | - |
dc.contributor.author | Cho, EJ[Cho, Eun-Jung] | - |
dc.contributor.author | Youn, HD[Youn, Hong-Duk] | - |
dc.date.accessioned | 2021-08-05T07:53:02Z | - |
dc.date.available | 2021-08-05T07:53:02Z | - |
dc.date.created | 2016-08-06 | - |
dc.date.issued | 2012-07-06 | - |
dc.identifier.issn | 1934-5909 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/64837 | - |
dc.description.abstract | O-linked-N-acetylglucosamine (O-GlcNAc) has emerged as a critical regulator of diverse cellular processes, but its role in embryonic stem cells (ESCs) and pluripotency has not been investigated. Here we show that O-GlcNAcylation directly regulates core components of the pluripotency network. Blocking O-GlcNAcylation disrupts ESC self-renewal and reprogramming of somatic cells to induced pluripotent stem cells. The core reprogramming factors Oct4 and Sox2 are O-GlcNAcylated in ESCs, but the O-GlcNAc modification is rapidly removed upon differentiation. O-GlcNAc modification of threonine 228 in Oct4 regulates Oct4 transcriptional activity and is important for inducing many pluripotency-related genes, including Klf2, Klf5, Nr5a2, Tbx3, and Tcl1. A T228A point mutation that eliminates this O-GlcNAc modification reduces the capacity of Oct4 to maintain ESC self-renewal and reprogram somatic cells. Overall, our study makes a direct connection between O-GlcNAcylation of key regulatory transcription factors and the activity of the pluripotency network. | - |
dc.publisher | CELL PRESS | - |
dc.subject | EMBRYONIC STEM-CELLS | - |
dc.subject | SELF-RENEWAL | - |
dc.subject | IN-VITRO | - |
dc.subject | ES C | - |
dc.title | O-GlcNAc Regulates Pluripotency and Reprogramming by Directly Acting on Core Components of the Pluripotency Network | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, EJ[Cho, Eun-Jung] | - |
dc.identifier.doi | 10.1016/j.stem.2012.03.001 | - |
dc.identifier.scopusid | 2-s2.0-84863622379 | - |
dc.identifier.wosid | 000306532400010 | - |
dc.identifier.bibliographicCitation | CELL STEM CELL, v.11, no.1, pp.62 - 74 | - |
dc.relation.isPartOf | CELL STEM CELL | - |
dc.citation.title | CELL STEM CELL | - |
dc.citation.volume | 11 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 62 | - |
dc.citation.endPage | 74 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ES C | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(03063) 25-2, SUNGKYUNKWAN-RO, JONGNO-GU, SEOUL, KOREAsamsunglib@skku.edu
COPYRIGHT © 2021 SUNGKYUNKWAN UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.