N-Acetylcysteine and N-Nitroarginine Methyl Ester Attenuate Carboplatin-Induced Ototoxicity in Dissociated Spiral Ganglion Neuron Cultures
DC Field | Value | Language |
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dc.contributor.author | Moon, IJ[Moon, Il Joon] | - |
dc.contributor.author | Kim, KR[Kim, Ki Ryung] | - |
dc.contributor.author | Chu, HS[Chu, Ho-Suk] | - |
dc.contributor.author | Kim, SH[Kim, Se Hyung] | - |
dc.contributor.author | Chung, WH[Chung, Won-Ho] | - |
dc.contributor.author | Cho, YS[Cho, Yang-Sun] | - |
dc.contributor.author | Hong, SH[Hong, Sung Hwa] | - |
dc.date.accessioned | 2021-08-06T02:49:46Z | - |
dc.date.available | 2021-08-06T02:49:46Z | - |
dc.date.created | 2016-08-06 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 1976-8710 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/70643 | - |
dc.description.abstract | Objectives. Carboplatin, a platinum-containing anti-cancer drug used to treat a variety of cancers, induces ototoxicity. Since, reactive oxygen species (ROS) and nitric oxide (NO) seem to be responsible for this toxicity, the antioxidant, N-acetyl-L-cysteine (L-NAC), and NO synthetase inhibitor, N-nitro-L-arginine methyl ester (L-NAME) were predicted to have protective effects against carboplatin ototoxicity. The aim of this study was to test for the protective effects of L-NAC and L-NAME on cochlear hair cells and spiral ganglion neurons (SGNs). Methods. Cochlear organotypic cultures and dissociated spiral ganglion neuron cultures, from mice postnatal day 5 cultures were used in this study. The cultures were treated with carboplatin alone or in combination with L-NAC or L-NAME, and carboplatin-induced damage was monitored. Results. Treatment with carboplatin induced a significant loss of outer hair cells, while inner hair cells were preserved in the cochlear organotypic cultures. Addition of L-NAC or L-NAME reduced the amount of carboplatin-induced hair cell damage; the differences did not reach statistical significance. However, carboplatin significantly decreased the number of surviving SGNs in dissociated cultures. The toxic effects were significantly reduced by addition of L-NAC or L-NAME. In addition, carboplatin induced the loss of neurites from the SGN somata, and this was not blocked with L-NAC or L-NAME. Conclusion. The results of this study suggest that ROS and NO are involved in carboplatin-induced damage to hair cells and SGNs, and administration of L-NAC/L-NAME can be used to attenuate the toxicity. | - |
dc.title | N-Acetylcysteine and N-Nitroarginine Methyl Ester Attenuate Carboplatin-Induced Ototoxicity in Dissociated Spiral Ganglion Neuron Cultures | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Moon, IJ[Moon, Il Joon] | - |
dc.contributor.affiliatedAuthor | Kim, KR[Kim, Ki Ryung] | - |
dc.contributor.affiliatedAuthor | Chu, HS[Chu, Ho-Suk] | - |
dc.contributor.affiliatedAuthor | Chung, WH[Chung, Won-Ho] | - |
dc.contributor.affiliatedAuthor | Cho, YS[Cho, Yang-Sun] | - |
dc.contributor.affiliatedAuthor | Hong, SH[Hong, Sung Hwa] | - |
dc.identifier.doi | 10.3342/ceo.2011.4.1.11 | - |
dc.identifier.scopusid | 2-s2.0-79953217376 | - |
dc.identifier.wosid | 000289052300002 | - |
dc.identifier.bibliographicCitation | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.4, no.1, pp.11 - 17 | - |
dc.relation.isPartOf | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | - |
dc.citation.title | CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY | - |
dc.citation.volume | 4 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 11 | - |
dc.citation.endPage | 17 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | other | - |
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