12-O-Tetradecanoyl phorbol-13-acetate (TPA)-induced growth arrest is increased by silibinin by the down-regulation of cyclin B1 and cdc2 and the up-regulation of p21 expression in MDA-MB231 human breast cancer cells
DC Field | Value | Language |
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dc.contributor.author | Kim, S[Kim, Sangmin] | - |
dc.contributor.author | Lee, HS[Lee, Hye Sook] | - |
dc.contributor.author | Lee, SK[Lee, Se-Kyung] | - |
dc.contributor.author | Kim, SH[Kim, Sung Hoon] | - |
dc.contributor.author | Hur, SM[Hur, Sung Mo] | - |
dc.contributor.author | Kim, JS[Kim, Jee Soo] | - |
dc.contributor.author | Kim, JH[Kim, Jung-Han] | - |
dc.contributor.author | Choe, JH[Choe, Jun-Ho] | - |
dc.contributor.author | Shin, I[Shin, Incheol] | - |
dc.contributor.author | Yang, JH[Yang, Jung-Hyun] | - |
dc.contributor.author | Lee, JE[Lee, Jeong Eon] | - |
dc.contributor.author | Nam, SJ[Nam, Seok Jin] | - |
dc.date.accessioned | 2021-08-06T08:49:34Z | - |
dc.date.available | 2021-08-06T08:49:34Z | - |
dc.date.created | 2016-10-20 | - |
dc.date.issued | 2010-12-01 | - |
dc.identifier.issn | 0944-7113 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/72572 | - |
dc.description.abstract | TPA is a potent regulator of cell growth, including cell proliferation and differentiation. In this study, we determined the effect of silibinin on TPA-induced growth arrest in breast cancer cells. Silibinin increased growth arrest of the G2/M phase in a dose-dependent fashion. Silibinin decreased the basal level of cyclin B1 and cdc2 expression, which is involved in S phase and G2/M transition. In addition, TPA-induced G2/M phase arrest was increased by silibinin. Under the same conditions, TPA-induced down-regulation of cyclin B1 and cdc2 was decreased by silibinin. In contrast, TPA-induced p21 expression was further increased by silibinin. To determine the regulatory mechanism of TPA-induced growth arrest, we pre-treated cells with various inhibitors, such as UO126, SB203580, and LY294002. Interestingly, TPA-induced growth arrest was significantly increased by LY294002, but not by UO126 and SB203580. In addition, TPA-induced down-regulation of cyclin B1 was inhibited by LY294002; however, the basal level of p21 was increased by TPA and TPA-induced p21 expression was further increased by LY294002. Finally, adenoviral constitutively active-Akt (Ad-CA-Akt) overexpression regulated the up-regulation of cyclin B1 and the down-regulation of p21. Therefore, we have demonstrated that silibinin has an additive effect on TPA-induced growth arrest through the PI-3-kinase/Akt-dependent pathway. (C) 2010 Elsevier GmbH. All rights reserved. | - |
dc.publisher | ELSEVIER GMBH, URBAN & FISCHER VERLAG | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | LUNG-CARCINOMA CELLS | - |
dc.subject | PHORBOL-ESTER | - |
dc.subject | INACTIVATION | - |
dc.subject | INHIBITION | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | PHOSPHATASE | - |
dc.subject | SURVIVAL | - |
dc.subject | P34CDC2 | - |
dc.subject | KAP | - |
dc.title | 12-O-Tetradecanoyl phorbol-13-acetate (TPA)-induced growth arrest is increased by silibinin by the down-regulation of cyclin B1 and cdc2 and the up-regulation of p21 expression in MDA-MB231 human breast cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, S[Kim, Sangmin] | - |
dc.contributor.affiliatedAuthor | Lee, SK[Lee, Se-Kyung] | - |
dc.contributor.affiliatedAuthor | Kim, SH[Kim, Sung Hoon] | - |
dc.contributor.affiliatedAuthor | Kim, JS[Kim, Jee Soo] | - |
dc.contributor.affiliatedAuthor | Kim, JH[Kim, Jung-Han] | - |
dc.contributor.affiliatedAuthor | Choe, JH[Choe, Jun-Ho] | - |
dc.contributor.affiliatedAuthor | Lee, JE[Lee, Jeong Eon] | - |
dc.contributor.affiliatedAuthor | Nam, SJ[Nam, Seok Jin] | - |
dc.identifier.doi | 10.1016/j.phymed.2010.03.013 | - |
dc.identifier.scopusid | 2-s2.0-78149416861 | - |
dc.identifier.wosid | 000286495600011 | - |
dc.identifier.bibliographicCitation | PHYTOMEDICINE, v.17, no.14, pp.1127 - 1132 | - |
dc.relation.isPartOf | PHYTOMEDICINE | - |
dc.citation.title | PHYTOMEDICINE | - |
dc.citation.volume | 17 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 1127 | - |
dc.citation.endPage | 1132 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | LUNG-CARCINOMA CELLS | - |
dc.subject.keywordPlus | PHORBOL-ESTER | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PHOSPHATASE | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | P34CDC2 | - |
dc.subject.keywordPlus | KAP | - |
dc.subject.keywordAuthor | Silibinin | - |
dc.subject.keywordAuthor | Silybum marianum | - |
dc.subject.keywordAuthor | TPA | - |
dc.subject.keywordAuthor | Cyclin B1 | - |
dc.subject.keywordAuthor | Cdc2 | - |
dc.subject.keywordAuthor | p21 | - |
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