Regulation of Blood Vessel versus Lymphatic Vessel Growth in the Cornea
DC Field | Value | Language |
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dc.contributor.author | Chung, ES[Chung, Eui-Sang] | - |
dc.contributor.author | Saban, DR[Saban, Daniel R.] | - |
dc.contributor.author | Chauhan, SK[Chauhan, Sunil K.] | - |
dc.contributor.author | Dana, R[Dana, Reza] | - |
dc.date.accessioned | 2021-08-07T03:57:49Z | - |
dc.date.available | 2021-08-07T03:57:49Z | - |
dc.date.created | 2016-10-20 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/78179 | - |
dc.description.abstract | PURPOSE. In the present study, the authors developed novel models to stimulate blood vessel formation (hemangiogenesis) versus lymphatic vessel formation (lymphangiogenesis) in the cornea. METHODS. Micropellets loaded with high-dose (80 ng) or low-dose (12.5 ng) basic fibroblast growth factor (bFGF) were placed in BALB/c corneas. Angiogenic responses were analyzed by immunohistochemistry to quantify blood neovessels (BVs) and lymphatic neovessels (LVs) to 3 weeks after implantation. Areas covered by BV and LV were calculated and expressed as a percentage of the total corneal area (percentage BV and percentage LV). Hemangiogenesis (HA) and lymphangiogenesis (LA) were also assessed after antibody blockade of VEGFR-2 or VEGFR-3 RESULTS. Although high-dose bFGF stimulation induced a more potent angiogenic response, the relative LV (RLV = percentage LV/percentage BV X 100) was nearly identical with high- and low-doses of bFGF. Delayed LA responses induced 3 weeks after implantation of high-dose bFGF resulted in a lymphatic vessel-dominant phenotype. Interestingly, the blockade of VEGFR-2 significantly suppressed BV and LV. However, the blockade of VEGFR-3 inhibited only LV (P = 0.0002) without concurrent inhibition of BV (P = 0.79), thereby resulting in a blood vessel-dominant phenotype CONCLUSIONS. An HA-dominant corneal phenotype can be obtained in BALB/c mice 2 weeks after implantation of an 80-ng bFGF micropellet with VEGFR-3 blockade. Alternatively, an LA-dominant corneal phenotype can be obtained 3 weeks after implantation of an 80-ng bFGF micropellet without supplementary modulating agents. These models will be useful in evaluating the differential contribution of BV and LV to a variety of corneal abnormalities, including transplant rejection, wound healing and microbial keratitis. (Invest Ophthalmol Vis Sci. 2009; 50: 1613-1618) DOI:10.1167/iovs.08-2212 | - |
dc.publisher | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | - |
dc.subject | FACTOR RECEPTOR-3 | - |
dc.subject | GRAFT-REJECTION | - |
dc.subject | MOUSE CORNEA | - |
dc.subject | IN-VITRO | - |
dc.subject | LYMPHANGIOGENESIS | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | VEGF | - |
dc.subject | NEOVASCULARIZATION | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | INFLAMMATION | - |
dc.title | Regulation of Blood Vessel versus Lymphatic Vessel Growth in the Cornea | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, ES[Chung, Eui-Sang] | - |
dc.identifier.doi | 10.1167/iovs.08-2212 | - |
dc.identifier.scopusid | 2-s2.0-64049106927 | - |
dc.identifier.wosid | 000264543400020 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.50, no.4, pp.1613 - 1618 | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.citation.title | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.citation.volume | 50 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1613 | - |
dc.citation.endPage | 1618 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | FACTOR RECEPTOR-3 | - |
dc.subject.keywordPlus | GRAFT-REJECTION | - |
dc.subject.keywordPlus | MOUSE CORNEA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | LYMPHANGIOGENESIS | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordPlus | NEOVASCULARIZATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
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