Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells
DC Field | Value | Language |
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dc.contributor.author | Yun, H[Yun, Heesu] | - |
dc.contributor.author | Im, HJ[Im, Hee-Jeong] | - |
dc.contributor.author | Choe, C[Choe, Chungyoul] | - |
dc.contributor.author | Roh, S[Roh, Sangho] | - |
dc.date.accessioned | 2022-06-28T00:51:03Z | - |
dc.date.available | 2022-06-28T00:51:03Z | - |
dc.date.created | 2022-06-28 | - |
dc.date.issued | 2022-07-01 | - |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/97900 | - |
dc.description.abstract | Lung cancer is the prominent cause of cancer-associated death primarily because of distant metastatic disease. The metastatic potential of non-small cell lung cancer (NSCLC) is associated with tumor cell aggregation. However, the systemic mechanotransduction mechanism by which tumor cells dynamically aggregate and disseminate is poorly understood, especially in NSCLC. In this study, we examine whether the cell surface matrix plays an important role in metastasis. We used poly-2-hydroxyethyl methacrylate-based 3D spheroid formation methods to mimic in vivo metastatic lesions. Supra-structural analysis of human NSCLC A549 cells stained with ruthenium red for transmission electron microscopy (TEM) showed that glycocalyx surrounding the cell surface in 2D culture decreases in 3D culture. Comprehensive gene expression analysis revealed that the genes associated with cell adhesion were distinctly enriched in A549 cell spheroids. Of these, downregulation of the tumor metastatic microenvironment facilitator LOXL2, a copper-dependent enzyme catalyzing posttranslational oxidative deamination of peptidyl lysine, was of special interest. Knockdown of LOXL2 thickened the cell surface matrix in 2D culture and impaired compact aggregate formation in 3D culture. Moreover, A549 cell spheroids with endogenous overexpression of LOXL2 increased their dissemination on basement extracellular matrix Matrigel. Overall, these data imply that cell detachment-downregulated LOXL2 contributes to cell surface matrix remodeling, leading to collective dissemination of free-floating aggregates. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | MOLECULAR-ORIGINS | - |
dc.subject | DISSEMINATION | - |
dc.subject | PROGRESSION | - |
dc.subject | GLYCOCALYX | - |
dc.subject | EXPRESSION | - |
dc.subject | BIOLOGY | - |
dc.subject | GROWTH | - |
dc.subject | GENE | - |
dc.title | Effect of LOXL2 on metastasis through remodeling of the cell surface matrix in non-small cell lung cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choe, C[Choe, Chungyoul] | - |
dc.contributor.affiliatedAuthor | Roh, S[Roh, Sangho] | - |
dc.identifier.doi | 10.1016/j.gene.2022.146504 | - |
dc.identifier.scopusid | 2-s2.0-85129001768 | - |
dc.identifier.wosid | 000800386500007 | - |
dc.identifier.bibliographicCitation | GENE, v.830 | - |
dc.relation.isPartOf | GENE | - |
dc.citation.title | GENE | - |
dc.citation.volume | 830 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | MOLECULAR-ORIGINS | - |
dc.subject.keywordPlus | DISSEMINATION | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | GLYCOCALYX | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | LOXL2 | - |
dc.subject.keywordAuthor | Cell surface glycocalyx | - |
dc.subject.keywordAuthor | Dissemination | - |
dc.subject.keywordAuthor | Metastasis | - |
dc.subject.keywordAuthor | NSCLC | - |
dc.subject.keywordAuthor | Lung cancer | - |
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