Homogeneous Electrochemical Assay for Protein Kinase Activity
- Authors
- Shin, Ik Soo; Chand, Rohit; Lee, Sang Wook; Rhee, Hyun-Woo; Kim, Yong-Sang; Hong, Jong-In
- Issue Date
- Nov-2014
- Publisher
- American Chemical Society
- Citation
- ANALYTICAL CHEMISTRY, v.86, pp.10992 - 10995
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 86
- Start Page
- 10992
- End Page
- 10995
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/10165
- DOI
- 10.1021/ac502549s
- ISSN
- 0003-2700
- Abstract
- Herein, we report a homogeneous assay for protein kinase activity using an electrochemistry-based probe. The approach involves a peptide substrate conjugated with a redox tag and the phosphate-specific receptor immobilized on an electrode surface. The peptide substrate phosphorylated by a protein kinase binds to the receptor site of the probe, which results in a redox current under voltammetric measurement. Our method was successfully applied even in the presence of citrated human blood and modified to enable a single-use, chip-based electrochemical assay for kinase activity.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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