Multielectrode Spectroscopy Enables Rapid and Sensitive Molecular Profiling of Extracellular Vesicles
- Authors
- Kilic, Tugba; Cho, Young Kwan; Jeong, Naebong; Shin, Ik-Soo; Carter, Bob S.; Balaj, Leonora; Weissleder, Ralph; Lee, Hakho
- Issue Date
- 26-Jan-2022
- Publisher
- AMER CHEMICAL SOC
- Citation
- ACS CENTRAL SCIENCE, v.8, no.1, pp.110 - 117
- Journal Title
- ACS CENTRAL SCIENCE
- Volume
- 8
- Number
- 1
- Start Page
- 110
- End Page
- 117
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42033
- DOI
- 10.1021/acscentsci.1c01193
- ISSN
- 2374-7943
- Abstract
- Detecting protein markers in extracellular vesicles (EVs) is becoming a useful tool for basic research and clinical diagnoses. Most EV protein assays, however, require lengthy processes-conjugating affinity ligands onto sensing substrates and affixing EVs with additional labels to maximize signal generation. Here, we present an iPEX (impedance profiling of extracellular vesicles) system, an all-electrical strategy toward fast, multiplexed rapidly functionalize sensor electrodes with antibodies; it then detects EV proteins in a label-free manner through impedance spectroscopy. The approach streamlines the entire EV assay, from sensor preparation to signal measurements. We achieved (i) fast immobilization of antibodies (<3 min) per electrode; (ii) high sensitivity (500 EVs/mL) without secondary labeling; and (iii) parallel detection (quadruple) in a single chip. A potential clinical utility was demonstrated by directly analyzing plasma samples from glioblastoma multiforme patients.
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- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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