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Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications

Authors
Kim, SuranChoi, Yi SunLee, Jung SeungJo, Sung-HyunKim, Yun-GonCho, Seung-Woo
Issue Date
25-Mar-2022
Publisher
ELSEVIER SCIENCE INC
Keywords
Decellularization; Intestinal extracellular matrix; Intestinal organoid; Hydrogel
Citation
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.107, pp.155 - 164
Journal Title
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
Volume
107
Start Page
155
End Page
164
URI
http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42121
DOI
10.1016/j.jiec.2021.11.044
ISSN
1226-086X
Abstract
Intestinal organoids are emerging tools in intestinal disease modeling and regeneration. Matrigel, a basement membrane matrix extracted from mouse sarcoma, is the gold standard material for intestinal organoid culture. Yet, the tumor origin of this matrix does not effectively provide organoids with native intestine-like microenvironments. To overcome this limitation, we demonstrate a decellularized small intestine-derived hydrogel reinforced with three extracellular matrix (ECM) proteins that are essential for intestinal development. To identify optimal conditions for intestinal organoid culture, we optimized the concentrations of the ECM hydrogel derived from decellularized intestine and three ECM proteins. The intestinal organoids grown in the established ECM hydrogels have shown similarity to those grown in Matrigel in terms of organoid forming efficiency, size, and gene expression patterns of intestinespecific markers. Our intestine-mimetic hydrogel could increase the feasibility of intestinal organoids for more clinically relevant applications in disease modeling and regenerative medicine. (c) 2021 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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