Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Suran | - |
dc.contributor.author | Choi, Yi Sun | - |
dc.contributor.author | Lee, Jung Seung | - |
dc.contributor.author | Jo, Sung-Hyun | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Cho, Seung-Woo | - |
dc.date.accessioned | 2022-04-13T01:40:23Z | - |
dc.date.available | 2022-04-13T01:40:23Z | - |
dc.date.created | 2022-04-13 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 1226-086X | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42121 | - |
dc.description.abstract | Intestinal organoids are emerging tools in intestinal disease modeling and regeneration. Matrigel, a basement membrane matrix extracted from mouse sarcoma, is the gold standard material for intestinal organoid culture. Yet, the tumor origin of this matrix does not effectively provide organoids with native intestine-like microenvironments. To overcome this limitation, we demonstrate a decellularized small intestine-derived hydrogel reinforced with three extracellular matrix (ECM) proteins that are essential for intestinal development. To identify optimal conditions for intestinal organoid culture, we optimized the concentrations of the ECM hydrogel derived from decellularized intestine and three ECM proteins. The intestinal organoids grown in the established ECM hydrogels have shown similarity to those grown in Matrigel in terms of organoid forming efficiency, size, and gene expression patterns of intestinespecific markers. Our intestine-mimetic hydrogel could increase the feasibility of intestinal organoids for more clinically relevant applications in disease modeling and regenerative medicine. (c) 2021 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.relation.isPartOf | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | - |
dc.title | Intestinal extracellular matrix hydrogels to generate intestinal organoids for translational applications | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jiec.2021.11.044 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.107, pp.155 - 164 | - |
dc.identifier.kciid | ART002825901 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000759157500001 | - |
dc.identifier.scopusid | 2-s2.0-85121253511 | - |
dc.citation.endPage | 164 | - |
dc.citation.startPage | 155 | - |
dc.citation.title | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | - |
dc.citation.volume | 107 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Decellularization | - |
dc.subject.keywordAuthor | Intestinal extracellular matrix | - |
dc.subject.keywordAuthor | Intestinal organoid | - |
dc.subject.keywordAuthor | Hydrogel | - |
dc.subject.keywordPlus | STEM-CELL | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | LAMININ | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | NEPHRONECTIN | - |
dc.subject.keywordPlus | FIBRONECTIN | - |
dc.subject.keywordPlus | GROWTH | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Soongsil University Library 369 Sangdo-Ro, Dongjak-Gu, Seoul, Korea (06978)02-820-0733
COPYRIGHT ⓒ SOONGSIL UNIVERSITY, ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.