Distinct cellular composition between normal surgical margins and tumor tissues in oral squamous cell carcinoma

Abstract

BackgroundAdequate resection of normal surgical margins is important. However, the clear distinction between the normal surgical margins and tumor tissues is still difficult.ObjectiveHere, this study analyzed the variety of cell types in tumors and the normal surgical margins using a computational approach.MethodsThe composition of cell types was compared between the two tissues by statistical and machine learning approaches.ResultsThe results showed the distinct cellular composition between tumor-adjacent and tumor tissues. In particular, endothelial cells were highly represented and macrophages were underrepresented at the normal surgical margin. Moreover, the normal surgical margin and tumor tissues could be discriminated using a machine learning algorithm.ConclusionThe results will help to understand cellular differences between normal surgical margins and tumor tissues and to provide potentials for tumor detection and treatment.