Electrochemiluminescent chemodosimeter based on iridium(III) complex for point-of-care detection of homocysteine levels
- Authors
- Kim, Hoon Jun; Lee, Kyung-Sik; Jeon, Yong-Jun; Shin, Ik-Soo; Hong, Jong-In
- Issue Date
- 15-May-2017
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Electrochemilurninescence (ECL); Homocysteine (Hcy); Chemodosimeter; Iridium complex
- Citation
- BIOSENSORS & BIOELECTRONICS, v.91, pp.497 - 503
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 91
- Start Page
- 497
- End Page
- 503
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/6370
- DOI
- 10.1016/j.bios.2017.01.010
- ISSN
- 0956-5663
- Abstract
- Elevated levels of plasma homocysteine (Hcy) are an independent risk factor for cardiovascular disease. Although a routine, rapid, and simple determination of Hcy levels is highly desired, the existing methods are practically limited because of complicated sample preparation and bulky instrumentation. Herein, we report a chemodosimetric approach for one-step analysis of Hey levels based on the electrochemiluminescence (ECL). A rationally designed cyclometalated iridium(III) complex possessing a phenylisoquinoline main ligand underwent a selective ring-formation reaction with Hcy to generate a binding adduct, which enabled producing highly luminescent excited states, and yielded strong ECL signals on the surface of electrode without any use of enzymes or antibodies. The level of Hey was successfully monitored by the ECL increment with a linear correlation between 0 and 40 mu M in 99.9% aqueous media. The approach required neither sample preparation nor bulky instrument, suggesting the point-of-care testing of Hey levels, and is potentially useful for routine, cost-effective, and precautionary diagnosis of various cardiovascular diseases.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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