Comparative N-Linked Glycan Analysis of Wild-Type and alpha 1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts Using Mass Spectrometry Approaches
- Authors
- Park, Hae-Min; Kim, Yoon-Woo; Kim, Kyoung-Jin; Kim, Young June; Yang, Yung-Hun; Jin, Jang Mi; Kim, Young Hwan; Kim, Byung-Gee; Shim, Hosup; Kim, Yun-Gon
- Issue Date
- Jan-2015
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- GalT-KO pig fibroblast; mass spectrometry (MS); N-glycan; N-glycolylneuraminic acid (NeuGc)
- Citation
- MOLECULES AND CELLS, v.38, no.1, pp.65 - 74
- Journal Title
- MOLECULES AND CELLS
- Volume
- 38
- Number
- 1
- Start Page
- 65
- End Page
- 74
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8839
- DOI
- 10.14348/molcells.2015.2240
- ISSN
- 1016-8478
- Abstract
- Carbohydrate antigens expressed on pig cells are considered to be major barriers in pig-to-human xenotransplantation. Even after alpha 1,3-galactosyltransferase gene knock-out (GalT-KO) pigs are generated, potential non-Gal antigens are still existed. However, to the best of our knowledge there is no extensive study analyzing N-glycans expressed on the GalT-KO pig tissues or cells. Here, we identified and quantified totally 47 N-glycans from wild-type (WT) and GalT-KO pig fibroblasts using mass spectrometry. First, our results confirmed the absence of galactose-alpha-1,3-galactose (alpha-Gal) residue in the GalT-KO pig cells. Interestingly, we showed that the level of overall fucosylated N-glycans from GalT-KO pig fibroblasts is much higher than from WT pig fibroblasts. Moreover, the relative quantity of the N-glycolylneuraminic acid (NeuGc) antigen is slightly higher in the GalT-KO pigs. Thus, this study will contribute to a better understanding of cellular glycan alterations on GalT-KO pigs for successful xenotransplantation.
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