Combined Poly(Lactide-Co-Glycolide) Microspheres Containing Diphtheria Toxoid for a Single-shot Immunization
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Woo, Hye Seung | - |
dc.contributor.author | Kim, Sung Rae | - |
dc.contributor.author | Yoon, Mikyeong | - |
dc.contributor.author | Lee, Eun Seok | - |
dc.contributor.author | Chang, In Ho | - |
dc.contributor.author | Whang, Young Mi | - |
dc.contributor.author | Lee, Do Ik | - |
dc.contributor.author | Kang, Myung Joo | - |
dc.contributor.author | Choi, Young Wook | - |
dc.date.available | 2019-01-22T13:29:54Z | - |
dc.date.issued | 2018-04 | - |
dc.identifier.issn | 1530-9932 | - |
dc.identifier.issn | 1522-1059 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1017 | - |
dc.description.abstract | To develop a single-shot vaccine containing diphtheria toxoid (DT) with a sufficient immune response, poly(lactide-co-glycolide) (PLGA) microspheres were prepared by water-in-oil-in-water double emulsification and solvent extraction techniques using low or high-molecular-weight PLGA (LMW-MS or HMW-MS). Stearic acid (SA) was introduced to HMW-MS (HMW/SA-MS) as a release modulator. Mean particle sizes (dvs, mu m) varied between the prepared microspheres, with LMW-MS, HMW-MS, and HMW/SA-MS having the sizes of 29.83, 110.59, and 69.5 mu m, respectively; however, the protein entrapment and loading efficiency did not vary, with values of 15.2-16.8 mu g/mg and 61-75%, respectively. LMW-MS showed slower initial release (similar to 2 weeks) but faster and higher release of antigen during weeks 3 similar to 7 than did HMW-MS. HMW/SA-MS showed rapid initial release followed by a continuous release over an extended period of time (similar to 12 weeks). Mixed PLGA microspheres (MIX-MS), a combination of HMW/SA-MS and LMW-MS (1:1), demonstrated a sufficient initial antigen release and a subsequent boost release in a pulsatile manner. Serum antibody levels were measured by ELISA after DT immunization of Balb/c mice, and showed a greater response to MIX-MS than to alum-adsorbed DT (control). A lethal toxin challenge test with MIX-MS (a DT dose of 18 Lf) using Balb/c mice revealed complete protection, indicating a good candidate delivery system for a single-shot immunization. | - |
dc.format.extent | 8 | - |
dc.publisher | SPRINGER | - |
dc.title | Combined Poly(Lactide-Co-Glycolide) Microspheres Containing Diphtheria Toxoid for a Single-shot Immunization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1208/s12249-017-0934-7 | - |
dc.identifier.bibliographicCitation | AAPS PHARMSCITECH, v.19, no.3, pp 1160 - 1167 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000428932700020 | - |
dc.identifier.scopusid | 2-s2.0-85037979844 | - |
dc.citation.endPage | 1167 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1160 | - |
dc.citation.title | AAPS PHARMSCITECH | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | PLGA | - |
dc.subject.keywordAuthor | single-shot vaccine | - |
dc.subject.keywordAuthor | diphtheria toxoid | - |
dc.subject.keywordAuthor | stearic acid | - |
dc.subject.keywordAuthor | immunization | - |
dc.subject.keywordPlus | IN-VITRO RELEASE | - |
dc.subject.keywordPlus | MHC CLASS-I | - |
dc.subject.keywordPlus | IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | PLGA MICROSPHERES | - |
dc.subject.keywordPlus | DELIVERY-SYSTEMS | - |
dc.subject.keywordPlus | VACCINE ANTIGENS | - |
dc.subject.keywordPlus | MODEL PROTEIN | - |
dc.subject.keywordPlus | MICROPARTICLES | - |
dc.subject.keywordPlus | TETANUS | - |
dc.subject.keywordPlus | DRUG | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.