목통의 페놀성 화합물의 항산화 활성 및 HPLC-UV 동시 함량분석
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 경민지 | - |
dc.contributor.author | 전혜진 | - |
dc.contributor.author | 황완균 | - |
dc.date.available | 2019-03-08T19:38:15Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0377-9556 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/10820 | - |
dc.description.abstract | Akebiae caulis have been used in folk medicines for diuretic, menstrual pain, and diuretic pain. It has been also resolved nephritis and cystitis. In this study, we isolated three phenolic compounds from 70% methanol extract using the open column chromatography. These isolated compounds which were 2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside, 5-O-caffeoylquinic acid, and syringoylglycerol 2-O-β-D-glucoside were determined by physico-chemical apparatus. Furthermore, we conducted DPPH and ABTS assay in order to screen the antioxidant activity of isolated three compounds. Also, we developed a rapidly HPLC-UV analysis method of two phenolic compounds (2-(3,4-dihydroxyphenyl)-ethyl-β-Dglucopyranoside, 5-O-caffeoylquinic acid) for evaluating the Akebiae Caulis collected 30 samples from different regions. From the experiments, all three isolated compounds showed the significant antioxidant activity. We suggested that the content criteria of marker compounds were shown by a simple and rapid HPLC-UV method. The contents respectively were 0.009% (2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside) and 0.036% (5-O-caffeoylquinic acid). | - |
dc.description.abstract | Akebiae caulis have been used in folk medicines for diuretic, menstrual pain, and diuretic pain. It has been also resolved nephritis and cystitis. In this study, we isolated three phenolic compounds from 70% methanol extract using the open column chromatography. These isolated compounds which were 2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside, 5-O-caffeoylquinic acid, and syringoylglycerol 2-O-β-D-glucoside were determined by physico-chemical apparatus. Furthermore, we conducted DPPH and ABTS assay in order to screen the antioxidant activity of isolated three compounds. Also, we developed a rapidly HPLC-UV analysis method of two phenolic compounds (2-(3,4-dihydroxyphenyl)-ethyl-β-Dglucopyranoside, 5-O-caffeoylquinic acid) for evaluating the Akebiae Caulis collected 30 samples from different regions. From the experiments, all three isolated compounds showed the significant antioxidant activity. We suggested that the content criteria of marker compounds were shown by a simple and rapid HPLC-UV method. The contents respectively were 0.009% (2-(3,4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside) and 0.036% (5-O-caffeoylquinic acid). | - |
dc.format.extent | 7 | - |
dc.publisher | 대한약학회 | - |
dc.title | 목통의 페놀성 화합물의 항산화 활성 및 HPLC-UV 동시 함량분석 | - |
dc.title.alternative | Anti-oxidant Activity of Phenolic Compounds and Simultaneous Contents Determination using HPLC-UV from the Akebiae caulis | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 약 학 회 지, v.59, no.3, pp 113 - 119 | - |
dc.identifier.kciid | ART002005560 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 119 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 113 | - |
dc.citation.title | 약 학 회 지 | - |
dc.citation.volume | 59 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Akebiae caulis | - |
dc.subject.keywordAuthor | 2-(3 | - |
dc.subject.keywordAuthor | 4-dihydroxyphenyl)-ethyl-β-D-glucopyranoside | - |
dc.subject.keywordAuthor | 5-O-caffeoylquinic acid | - |
dc.subject.keywordAuthor | syringoylglycerol 2-O-β-D-glucoside | - |
dc.subject.keywordAuthor | antioxidant activity | - |
dc.subject.keywordAuthor | HPLC-UV | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.