Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures
DC Field | Value | Language |
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dc.contributor.author | Kim, S.H. | - |
dc.contributor.author | Lee, W.S. | - |
dc.contributor.author | Lee, Na Mi | - |
dc.contributor.author | Chae, Soo Ahn | - |
dc.contributor.author | Yun, Sin Weon | - |
dc.date.available | 2019-03-08T20:36:59Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 1738-1061 | - |
dc.identifier.issn | 2092-7258 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11322 | - |
dc.description.abstract | Purpose: The aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain. Methods: We prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis. Results: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P =0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P =0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P =0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P =0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P = 0.004). Conclusion: Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain. © 2015 by The Korean Pediatric Society. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Pediatric Society | - |
dc.title | Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures | - |
dc.type | Article | - |
dc.identifier.doi | 10.3345/kjp.2015.58.4.142 | - |
dc.identifier.bibliographicCitation | Korean Journal of Pediatrics, v.58, no.4, pp 142 - 147 | - |
dc.identifier.kciid | ART001982877 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.scopusid | 2-s2.0-84927749428 | - |
dc.citation.endPage | 147 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 142 | - |
dc.citation.title | Korean Journal of Pediatrics | - |
dc.citation.volume | 58 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Brain | - |
dc.subject.keywordAuthor | Hippocampus | - |
dc.subject.keywordAuthor | Hypoxia | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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