Gold nanoparticle-DNA aptamer composites as a universal carrier for in vivo delivery of biologically functional proteins
DC Field | Value | Language |
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dc.contributor.author | Ryou, Sang-Mi | - |
dc.contributor.author | Yeom, Ji-Hyun | - |
dc.contributor.author | Kang, Hyo Jung | - |
dc.contributor.author | Won, Miae | - |
dc.contributor.author | Kim, Jin-Sik | - |
dc.contributor.author | Lee, Boeun | - |
dc.contributor.author | Seong, Maeng-Je | - |
dc.contributor.author | Ha, Nam-Chul | - |
dc.contributor.author | Bae, Jeehyeon | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.available | 2019-03-08T20:40:21Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.issn | 1873-4995 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11476 | - |
dc.description.abstract | Although the delivery of biologically functional protein(s) into mammalian cells could be of tremendous value to biomedical research, the development of such technology has been hindered by the lack of a safe and effective delivery method. Here, we present a simple, efficient, and versatile gold nanoparticle-DNA aptamer conjugate (AuNP-Apt)-based system, with nanoblock-like properties, that allows any recombinant protein to be loaded without additional modifications and delivered into mammalian living systems. AuNP-Apt-based protein delivery system was able to deliver various proteins into variety of cell types in vitro without showing cytotoxicity. This AuNP-Apt system was also effective for the local and systemic targeted delivery of proteins in vivo. A local injection of the AuNP-Apt loaded with the apoptosis-inducing BIM protein efficiently inhibited the growth of xenograft tumors in mice. Furthermore, an intravenous injection of AuNP-Apt loaded with both epidermal growth factor (EGF) and BIM resulted in the targeted delivery of BIM into a xenograft tumor derived from EGF receptor-overexpressing cancer cells with no detectable systemic toxicity. Our findings show that this system can serve as an innovative platform for the development of protein-based biomedical applications. (C) 2014 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Gold nanoparticle-DNA aptamer composites as a universal carrier for in vivo delivery of biologically functional proteins | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jconrel.2014.10.021 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.196, pp 287 - 294 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000346132100030 | - |
dc.identifier.scopusid | 2-s2.0-84908457645 | - |
dc.citation.endPage | 294 | - |
dc.citation.startPage | 287 | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 196 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Protein delivery | - |
dc.subject.keywordAuthor | Targeted delivery | - |
dc.subject.keywordPlus | COPOLYMER MICELLES | - |
dc.subject.keywordPlus | TARGETED DELIVERY | - |
dc.subject.keywordPlus | LIGAND | - |
dc.subject.keywordPlus | TUMORS | - |
dc.subject.keywordPlus | NANOCARRIERS | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | BIM | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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