Metabolism of Kaempferia parviflora Polymethoxyflavones by Human Intestinal Bacterium Bautia sp MRG-PMF1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mihyang | - |
dc.contributor.author | Kim, Nayoung | - |
dc.contributor.author | Han, Jaehong | - |
dc.date.available | 2019-03-08T20:40:25Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.issn | 1520-5118 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11478 | - |
dc.description.abstract | Poylmethoxyflavones (PMFs) are major bioactive flavonoids, which exhibit various biological activities, such as anticancer effects. The biotransformation of PMFs and characterization of a PMF-metabolizing human intestinal bacterium were studied herein for the first time. Hydrolysis of aryl methyl ether functional groups by human fecal samples was observed from the bioconversion of various PMFs. Activity-guided screening for PMF-metabolizing intestinal bacteria under anaerobic conditions resulted in the isolation of a strict anaerobic bacterium, which was identified as Blautia sp. MRG-PMF1. The isolated MRG-PMF1 was able to metabolize various PMFs to the corresponding demethylated flavones. The microbial conversion of bioactive 5,7-dimethoxyflavone (5,7-DMF) and 5,7,4'-trimethoxyflavone (5,7,4'-TMF) was studied in detail. 5,7-DMF and 5,7,4'-TMF were completely metabolized to 5,7-dihydroxyflavone (chrysin) and 5,7,4'-trihydroxyflavone (apigenin), respectively. From a kinetics study, the methoxy group on the flavone C-7 position was found to be preferentially hydrolyzed. 5-Methoxychrysin, the intermediate of 5,7-DMF metabolism by Blautia sp. MRG-PMF1, was isolated and characterized by nuclear magnetic resonance spectroscopy. Apigenin was produced from the sequential demethylation of 5,7,4'-TMF, via 5,4'-dimethoxy-7-hydroxyflavone and 7,4'-dihydroxy-5-methoxyflavone (thevetiaflavone). Not only demethylation activity but also deglycosylation activity was exhibited by Blautia sp. MRG-PMF1, and various flavonoids, including isoflavones, flavones, and flavanones, were found to be metabolized to the corresponding aglycones. The unprecedented PMF demethylation activity of Blautia sp. MRG-PMF1 will expand our understanding of flavonoid metabolism in the human intestine and lead to novel bioactive compounds. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Metabolism of Kaempferia parviflora Polymethoxyflavones by Human Intestinal Bacterium Bautia sp MRG-PMF1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/jf504074n | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.51, pp 12377 - 12383 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000347138500013 | - |
dc.identifier.scopusid | 2-s2.0-84919779924 | - |
dc.citation.endPage | 12383 | - |
dc.citation.number | 51 | - |
dc.citation.startPage | 12377 | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 62 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | biotransformation | - |
dc.subject.keywordAuthor | Balutia | - |
dc.subject.keywordAuthor | demethylation | - |
dc.subject.keywordAuthor | human intestinal bacterial | - |
dc.subject.keywordAuthor | polymethoxyflavone | - |
dc.subject.keywordPlus | LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | PHENOLIC-COMPOUNDS | - |
dc.subject.keywordPlus | GUT MICROBIOTA | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PHARMACOKINETICS | - |
dc.subject.keywordPlus | BIOAVAILABILITY | - |
dc.subject.keywordPlus | (3S)-EQUOL | - |
dc.subject.keywordPlus | EXTRACTS | - |
dc.subject.keywordPlus | HEALTH | - |
dc.subject.keywordPlus | URINE | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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