Signal Enhancement for Ultrasensitive Detection of Versatile Biomaterials Using Specific Aptamer Modified Au Nano-Complex
DC Field | Value | Language |
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dc.contributor.author | Choi, Jin-Ha | - |
dc.contributor.author | Jang, Hye-Mi | - |
dc.contributor.author | Mo, Kyosung | - |
dc.contributor.author | Um, Soong Ho | - |
dc.contributor.author | Min, Junhong | - |
dc.contributor.author | Oh, Byung-Keun | - |
dc.date.available | 2019-03-08T20:58:34Z | - |
dc.date.issued | 2014-11 | - |
dc.identifier.issn | 1947-2935 | - |
dc.identifier.issn | 1947-2943 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11695 | - |
dc.description.abstract | For early diagnosis of several diseases, highly sensitive detection technology is necessary to analyze biomarkers quantitatively at low concentrations. To develop a sensitive detection system, a well-designed probe is very critical, and it mainly consisted of several bio-and nanomaterials, such as gold (Au) and silica nanoparticles. In this study, an ultrasensitive nanoprobe for the detection of versatile biomolecules was developed by the sandwich immuno-reaction technique. The developed nanoprobe was composed of aptamer-functionalized Au nanoparticles and magnetic microparticles (MMPs). In order to enhance the detection ability of the Au probe, the orientation of antibodies was defined using aptamer-induced specific binding to the F-c region of the antibody. And target antigens could be detected by the real-time polymerase chain reaction (RT-PCR) method, which was able to measure the concentration of aptamH7 (E. coli O157:H7). This system can be applied to the diagnosis of several diseases and environmental toxicity testing, especially for the detection of very low concentrations. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.title | Signal Enhancement for Ultrasensitive Detection of Versatile Biomaterials Using Specific Aptamer Modified Au Nano-Complex | - |
dc.type | Article | - |
dc.identifier.doi | 10.1166/sam.2014.2239 | - |
dc.identifier.bibliographicCitation | SCIENCE OF ADVANCED MATERIALS, v.6, no.11, pp 2547 - 2553 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000348431100049 | - |
dc.identifier.scopusid | 2-s2.0-84920286971 | - |
dc.citation.endPage | 2553 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2547 | - |
dc.citation.title | SCIENCE OF ADVANCED MATERIALS | - |
dc.citation.volume | 6 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Immuno-PCR | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Au Nanoparticle | - |
dc.subject.keywordPlus | SURFACE-PLASMON RESONANCE | - |
dc.subject.keywordPlus | CLEAVAGE REACTION | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ELISA | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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