Simultaneous determination of 7-O-succinyl macrolactin A and its active major metabolite, macrolactin A in dog plasma using high-performance liquid chromatography with UV detection
DC Field | Value | Language |
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dc.contributor.author | Kim, Eunyoung | - |
dc.contributor.author | Shin, Beomsoo | - |
dc.contributor.author | Kwon, Kwang-il | - |
dc.contributor.author | Bang, Joon Seok | - |
dc.contributor.author | Kang, Wonku | - |
dc.date.available | 2019-03-08T21:00:10Z | - |
dc.date.issued | 2014-10 | - |
dc.identifier.issn | 1615-9306 | - |
dc.identifier.issn | 1615-9314 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11774 | - |
dc.description.abstract | We developed a method for the simultaneous quantification of 7-O-succinyl macrolactin A and its active metabolite, macrolactin A, in dog plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard, 7-O-succinyl macrolactin A, macrolactin A, and flufenamic acid were chromatographed on a reverse-phase C-18 analytical column. The mobile phase, consisting of 20 mM acetate buffer and acetonitrile, was eluted using a gradient program at 1 mL/min, and the UV absorbance was measured at 230 nm. The retention times of 7-O-succinylmacrolactin A, flufenamic acid, and macrolactin A were 3.4, 4.8, and 6.9 min, respectively. The coefficient of variation in the assay precision for both substances was less than 6%, and the accuracy ranged from 96 to 105%. This method was used to measure the concentrations of 7-O-succinylmacrolactin A and macrolactin A in dog plasma following an intravenous administration of a single dose (25 mg/kg) of 7-O-succinyl macrolactin A salt. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Simultaneous determination of 7-O-succinyl macrolactin A and its active major metabolite, macrolactin A in dog plasma using high-performance liquid chromatography with UV detection | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jssc.201400438 | - |
dc.identifier.bibliographicCitation | JOURNAL OF SEPARATION SCIENCE, v.37, no.20, pp 2833 - 2836 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000343819300004 | - |
dc.identifier.scopusid | 2-s2.0-84907965731 | - |
dc.citation.endPage | 2836 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 2833 | - |
dc.citation.title | JOURNAL OF SEPARATION SCIENCE | - |
dc.citation.volume | 37 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Dog plasma | - |
dc.subject.keywordAuthor | High-performance liquid chromatography | - |
dc.subject.keywordAuthor | Macrolactin A | - |
dc.subject.keywordAuthor | 7-O-Succinyl macrolactin A | - |
dc.subject.keywordAuthor | UV detection | - |
dc.subject.keywordPlus | VANCOMYCIN-RESISTANT ENTEROCOCCI | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | RAT PLASMA | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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