Arsenite-induced changes in hepatic protein abundance in cynomolgus monkeys (Macaca fascicularis)
DC Field | Value | Language |
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dc.contributor.author | Kim, Soohee | - |
dc.contributor.author | Lee, Seung Heon | - |
dc.contributor.author | Lee, Seungwoo | - |
dc.contributor.author | Park, Jung-Duck | - |
dc.contributor.author | Ryu, Doug-Young | - |
dc.date.available | 2019-03-08T21:38:28Z | - |
dc.date.issued | 2014-08 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.issn | 1615-9861 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11977 | - |
dc.description.abstract | Arsenic is an environmental pollutant, and its liver toxicity has long been recognized. The effect of arsenic on liver protein expression was analyzed using a proteomic approach in monkeys. Monkeys were orally administered sodium arsenite (SA) for 28 days. As shown by 2D-PAGE in combination with MS, the expression levels of 16 proteins were quantitatively changed in SA-treated monkey livers compared to control-treated monkey livers. Specifically, the levels of two proteins, mortalin and tubulin beta chain, were increased, and 14 were decreased, including plastin-3, cystathionine-beta-synthase, selenium-binding protein 1, annexin A6, alpha-enolase, phosphoenolpyruvate carboxykinase-M, erlin-2, and arginase-1. In view of their functional roles, differential expression of these proteins may contribute to arsenic-induced liver toxicity, including cell death and carcinogenesis. Among the 16 identified proteins, four were selected for validation by Western blot and immunohistochemistry. Additional Western blot analyses indicated arsenic-induced dysregulation of oxidative stress related, genotoxicity-related, and glucose metabolism related proteins in livers from SA-treated animals. Many changes in the abundance of toxicity-related proteins were also demonstrated in SA-treated human hepatoma cells. These data on the arsenic-induced regulation of proteins with critical roles may help elucidate the specific mechanisms underlying arsenic-induced liver toxicity. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Arsenite-induced changes in hepatic protein abundance in cynomolgus monkeys (Macaca fascicularis) | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/pmic.201300509 | - |
dc.identifier.bibliographicCitation | PROTEOMICS, v.14, no.15, pp 1833 - 1843 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000340398600011 | - |
dc.identifier.scopusid | 2-s2.0-84905284074 | - |
dc.citation.endPage | 1843 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 1833 | - |
dc.citation.title | PROTEOMICS | - |
dc.citation.volume | 14 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Animal proteomics | - |
dc.subject.keywordAuthor | Arsenic | - |
dc.subject.keywordAuthor | Liver | - |
dc.subject.keywordAuthor | Monkey | - |
dc.subject.keywordAuthor | Toxicity | - |
dc.subject.keywordPlus | RNA DIFFERENTIAL DISPLAY | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | SODIUM ARSENITE | - |
dc.subject.keywordPlus | PROTECTIVE ROLE | - |
dc.subject.keywordPlus | T-PLASTIN | - |
dc.subject.keywordPlus | GLUTATHIONE-PEROXIDASE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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