Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1
DC Field | Value | Language |
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dc.contributor.author | Pham Anh Tuan | - |
dc.contributor.author | Kwon, Do Yeon | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Arasu, Mariadhas Valan | - |
dc.contributor.author | Al-Dhabi, Naif Abdullah | - |
dc.contributor.author | Park, Nam Il | - |
dc.contributor.author | Park, Sang Un | - |
dc.date.available | 2019-03-08T21:39:11Z | - |
dc.date.issued | 2014-08 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12008 | - |
dc.description.abstract | To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines showing over-expression of AtPAP1 were generated. In order to investigate the regulation of AtPAP1 on the activities of CGA biosynthetic genes, the expression levels of seven P. grandiflorum CGA biosynthetic genes were analyzed in the hairy root line that had the greatest accumulation of AtPAP1 transcript, OxPAP1-1. The introduction of AtPAP1 increased the mRNA levels of all examined CGA biosynthetic genes and resulted in a 900% up-regulation of CGA accumulation in OxPAP1-1 hairy roots relative to controls. This suggests that P. grandiflorum hairy roots that over-express the AtPAP1 gene are a potential alternative source of roots for the production of CGA. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms150814743 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.15, no.8, pp 14743 - 14752 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000341519600096 | - |
dc.identifier.scopusid | 2-s2.0-84921053048 | - |
dc.citation.endPage | 14752 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 14743 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 15 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Agrobacterium rhizogenes | - |
dc.subject.keywordAuthor | chlorogenic acid | - |
dc.subject.keywordAuthor | gene over-expression | - |
dc.subject.keywordAuthor | hairy root | - |
dc.subject.keywordAuthor | Platycodon grandiflorum | - |
dc.subject.keywordAuthor | transcription factor | - |
dc.subject.keywordAuthor | production of anthocyanin pigment 1 | - |
dc.subject.keywordPlus | PHENYLPROPANOID BIOSYNTHESIS | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | PLANTS | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | COFFEE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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