Regeneration Effect of Visible Light-Curing Furfuryl Alginate Compound by Release of Epidermal Growth Factor for Wound Healing Application

Abstract

A visible light-curable polysaccharide conjugate was prepared and was included with epidermal growth factor (EGF) for wound healing application. Alginate was modified with furfurylamine, and the furfuryl alginate (F-alginate) was mixed with Rose Bengal for visible light reactivity. The introduction of furfuryl group was confirmed by H-1-NMR and UV/vis spectroscopy. Visible light-induced crosslinking was evaluated by micropatterning and weight measurement. Swelling ratio was also determined. To verify its applicability in wound healing applications, EGF was included in the system of F-alginate and Rose Bengal. After the confirmation of no cytotoxicity of F-alginate and murine EGF release from the system, cell proliferation, antibacterial activity, and animal test were performed on the system. In conclusion, it was demonstrated that the system was useful for wound healing. (C) 2013 Wiley Periodicals, Inc.